*2.10. BaV, But Not Kn-Ba, Induced the Production of PGE2*

The concentrations of the eicosanoids PGE2 and LTB4 were evaluated in cell-free supernatants by competitive ELISA after 30 min of treatments with higher doses of BaV (15 μg/well) and Kn-Ba (1 μg/well). BaV, but not Kn-Ba, induced the production of PGE2 (Figure 7). No stimulus was able to induce the production of LTB4 (data not shown).

**Figure 7.** Production of PGE2. THP-1 macrophages (2 <sup>×</sup> <sup>10</sup><sup>5</sup> cells/well) were treated with higher concentrations of BaV (15 μg/well) and Kn-Ba (1 μg/well). Cells incubated only with culture medium were used as control. After 30 min, the production of PGE2 was evaluated by ELISA. Results expressed as mean of duplicates ± SEM and analyzed by one-way ANOVA followed by Tukey's post-test. (Asterisk) Significant difference from control and Kn-Ba. *p* < 0.01 (\*\*).
