*5.9. Statistical Analysis*

The GraphPad Prism software was used (version 5.0). Student's *t*-test and one-way ANOVA (followed by Tukey's post test) were used for comparisons, and differences were considered significant at *p* < 0.05.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/10 .3390/toxins13110830/s1, Figure S1: Myofibroblast differentiation in Calu-3 conditioned medium. Figure S2: MRC-5/Calu-3 spheroid. Figure S3: Invasion area of MRC-5/Calu-3 spheroids in 3D collagen gels. Figure S4: Expression of EMT-related proteins.

**Author Contributions:** All authors contributed to data analysis, model development, and manuscript writing. Thus, each author participated sufficiently in the work to take public responsibility for appropriate portions of the content and, therefore, agreed to be accountable for all aspects in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by São Paulo Research Foundation (FAPESP)-Grants 2017/11161- 6. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. This study was financed in part by the National Council for Scientific and Technological Development—CNPq-Productivity grant (301685/2017-7).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** The authors gratefully acknowledge the financial support by São Paulo Research Foundation, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors also thank, for technical support, Alexsander S. de Souza from the Multiuser Platform of the Scientific Development Center of the Butantan Institute, São Paulo, Brazil, for confocal microscopy.

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
