*5.11. Statistical Analysis*

Statistical analysis was performed using GraphPad Prism version 6 software (Graph-Pad, San Diego, CA, USA). Test one-way analysis of variance (ANOVA) followed by Tukey's post-test was used for comparisons among three or more groups. Two-way analysis of variance (ANOVA) followed by Bonferroni post-test was used to compare repeated measures of the electronic von Frey test. The data are expressed as the mean ± SEM and *p* < 0.05 was indicative of a significant statistical difference.

**Author Contributions:** Conceptualization, G.P., V.O.Z., M.C. and R.L.P.; methodology, A.C.G., B.G.E., M.B.S., B.B.M., histological and immune assays, A.C.G., B.G.E., C.L.P.L., A.P.C., O.G.R., formal analysis, A.C.G., M.B.S., G.P., V.O.Z., M.C., R.L.P.; data curation, A.C.G., B.G.E., B.B.M., writing original draft preparation, A.C.G.; supervision, G.P.; funding acquisition, G.P., V.O.Z., M.C., R.L.P. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by funds from Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq (Process number 467211/2014-0), from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil [CAPES- Finance Code 001] and from São Paulo Research Foundation (FAPESP) grants 2015/01254-1 and 2013/07467-1 (CETICs program).

**Institutional Review Board Statement:** All procedures were performed according to the "Ethical Guide for the Use of Pain-Sensitive Animals in Pain Testing", published by IASP [93], reported in accordance with the Animal Research Reporting of In Vivo Experiments (ARRIVE) guidelines (http://www.nc3rs.org.uk/arrive-guidelines, accessed on 27 October 2021) and approved by the Ethics Committee on Animal Use of the Butantan Institute (CEUAIB protocol number 7334170718, approval date 7 August 2018).

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Conflicts of Interest:** The authors declare no conflict of interest.
