*4.4. Enzymatic Deglycosylation*

In denaturing conditions, for protein deglycosylation, toxin (3.4 μg) were set in 20% SDS for 1 min at 95 ◦C. After adding 0.2 M sodium phosphate buffer, 0.08% sodium azide, 0.5 M EDTA pH 8, 10% nonidet P-40, incubation was prolonged for 2 min at 95 ◦C. After cooling, 1 U.μL−<sup>1</sup> of N-glycosidase F (Roche, Mannheim, Germany) or 25 mU.50 μL−<sup>1</sup> O-glycosidase was added, and the mixture was incubated for 18 h at 37 ◦C. The deglycosylation profiles were evaluated by SDS-PAGE.
