*5.3. Total Protein Quantification*

Total protein concentration of BaV and Kn-Ba were measured by the bicinchoninic acid method [65] using the Pierce BCA Protein Assay kit (Pierce Biotechnology, Waltham, IL, USA), according to manufacture instructions. A standard curve was prepared using increased concentrations (0–2000 μg/mL) of pure bovine serum albumin (BSA, Sigma Aldrich, St. Louis, MO, USA) diluted in sterile PBS pH 7.4. Absorbances were obtained in the plate spectrophotometer (ELX 800, Biotek Instruments, Winooski, VT, USA) at ᐬ540 nm.
