*5.5. Statistical Analyses*

Statistical analysis was performed using GraphPad Prism 8 program (GraphPad Software Inc., San Diego, CA, USA). The results are presented as the mean ± SEM. The statistical evaluation of the data was performed using two-way analysis of variance (ANOVA) with post hoc testing by Tukey for Figures 1, 3, 4 and 5B. Figures 2 and 5D,E were analyzed using One-way ANOVA followed by Tukey's post hoc test. A value of *p* < 0.05 was considered significant.

**Author Contributions:** Conceptualization, V.O.Z. and Y.C.; behavior experiments, B.G.d.F., N.G.H. and L.M.P., western blot experiments T.C.B., N.G.H. and B.G.d.F.; data analysis, B.G.d.F., N.G.H. and V.O.Z.; writing—original draft preparation, N.G.H. and V.O.Z.; writing—review and editing, V.O.Z. and G.P.; supervision, V.O.Z.; project administration, V.O.Z.; funding acquisition, V.O.Z. and Y.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by funds from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) grants numbers 305345/2011-7 and 444299/2014-9; Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (FAPESP) grants numbers 2011/04459-2 and 2013/07467-1 (CETICs program); and the Instituto Nacional de Ciência e Tecnologia em Toxinologia (INCTTOX PROGRAM) (CNPq) grant number 573790/2008-6/FAPESP (grant number 2008/57898-0). BGF and LMP received a fellowship from the CNPq/PIBIC (144228/2014-9 and 107340/2005-4, respectively). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)— Finance Code 001. Fundaçao Butantan. ¯

**Institutional Review Board Statement:** The study was conducted according to the guidelines Ethics Committee on the Use of Experimental Animals at the Butantan Institute (protocol number 1245/14 (date of approval 17 April 2019) and 9766020419 (date of approval 19 March 2014)).

**Informed Consent Statement:** Not applicable.

**Acknowledgments:** Schematic images created with BioRender.com.

**Conflicts of Interest:** Authors declare no conflict of interest.
