*2.5. Hierarchical Clustering Analysis*

Hierarchical clustering analysis of differentially expressed proteins with FC ≥ 1.5 identified in both MCF7 and MDA-MB-231 cell lines characterized those proteins in seven major clusters (Figure 3, Table S3). Clusters 1–4 had proteins with fold change (FC) increased at 2.5 μg/mL of venom treatment in MCF7 cells and presented no change in MDA-MB-231 cell lines. Proteins in these clusters include HEL-S-156 and PRIC295 in cluster 1 and PSMD5 and PSME2 in cluster 2. Cluster 5 identified proteins that decreased in FC at 2.5 μg/mL of venom treatment in both cell lines including HIST1H4J, VDAC1, and VDAC2. Cluster 6 identified proteins that increased FC at 2.5 μg/mL of venom treatment in MDA-MB-231 cell line including H3F3B, LAP3, and KRT1, and cluster 7 identified proteins that did not change when cells were treated with low and high venom treatment, but they presented higher FC change in MDA-MB-231 when compared to MCF7 cell line such as RPS29 (Table 1).
