*5.2. Cell Culture*

The human non-small cell lung adenocarcinoma A549 cell line was kindly provided by Dr Durvanei Augusto Maria, Laboratory of Biochemistry and Biophysics, Butantan Institute. MRC-5 human lung fibroblast cell lines were purchased from Rio de Janeiro Bank Cells (Banco de Células do Rio de Janeiro, BCRJ code-0180) (Rio de Janeiro, Brazil). Monolayer cell was cultured in 75 cm<sup>2</sup> flasks, containing DMEM (Dulbecco's Modified Eagles Medium) supplemented with 10% fetal bovine serum (FBS), 100 units/mL of penicillin, and 0.1 mg/mL of streptomycin (CultiLab) with 5% CO2 at 37 ◦C for 48 h. Subconfluent cells were trypsinized with trypsin/EDTA (Gibco®) for subsequent use. A549 cells from passage 32–37, MRC-5 cells from passage 29–32 were used in our experiments.
