*5.7. Proteome Functional and Enrichment Analysis*

The entire proteome was analyzed to classify protein profiles by biological processes, molecular function, cellular components, and cellular pathways using Gene Ontology (geneontology.org accessed on 1 October 2019) and PantherDB (pantherdb.org accessed on 1 October 2019). The proteins presenting different profiles in the venom conditions were also analyzed using KEGG (www.genome.jp/kegg accessed on 1 October 2019) canonical pathways to determine the protein group or pathways that have undergone changes with different concentrations of the venom treatment. The PANTHER Classification System (Protein ANalysis THrough Evolutionary Relationships) is a research tool that covers evolutionary and functional information on protein family genes and has been widely used to understand protein evolution and its functional classification [148,149]. We conducted the functional classification for the molecular function, cell component, biological process, and protein family classification profiles [150,151] of the different proteins in each cell line and treatment at low and high *B. jararaca* venom that presented FC ≥ 1.5 in order to identify an enrichment pattern of protein families and pathways affected by the venom in the cell lines.
