*5.6. Statistical Analysis*

The results were statistically analyzed using the GraphPad Prism 5 program, using the one-way ANOVA test followed by Tukey's post-test. Results with a *p*-value < 0.05 were considered significant.

**Author Contributions:** Conceptualization, B.D. and F.C.V.P.; methodology, B.D., D.C.-C., C.C.S.-B., C.C.F.S. and R.T.K.; validation, B.D., C.C.S.-B., C.C.F.S. and F.C.V.P.; formal analysis, B.D., D.C.-C., C.C.S.-B., C.C.F.S., R.T.K. and F.C.V.P.; investigation, B.D., C.C.S.-B. and C.C.F.S.; resources, F.C.V.P.; data curation, B.D., D.C-C., C.C.S.-B., C.C.F.S. and R.T.K.; writing—original draft preparation, B.D., D.C.-C., C.C.S.-B., C.C.F.S., R.T.K. and F.C.V.P.; writing—review and editing, B.D., D.C.-C., C.C.S.-B., C.C.F.S., R.T.K. and F.C.V.P.; supervision, F.C.V.P.; project administration, F.C.V.P.; funding acquisition, B.D., D.C.-C., R.T.K. and F.C.V.P. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by grants from FAPESP (BD: 2014/12976-5, DC-C: 2013/15343-0, RTK: 2015/13124-5, FCVP: 2019/20832-7) and CAPES (CCFS: 88882.377116/2019-01).

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Institutional Review Board (or Ethics Committee) of Butantan Institute (CEUAIB protocol number 5396310517, approved on 21 June 2017).

**Informed Consent Statement:** Not applicable.

**Acknowledgments:** The authors thank Martin Wesley for English reviewing in the article.

**Conflicts of Interest:** The authors declare that they have no competing financial interest.

### **References**

