*5.1. Bacterial Strains, Plasmids, and Antigen*

The bacterial Phage-resistant *Escherichia coli* Omnimax (Invitrogen) was used for Phage Display assays. For Fab cloning and expression, *Escherichia coli* DH5a (Thermo Fisher Scientific) and BL21(DE3) pLysS (Novagen) were used, respectively. The plasmid vectors used were phagemid HP153 and pFabHis-MBP [25]. The bacterial strains used in this study were strains previously defined as STEC by gene presence and Stx1 or Stx2 production [34], including the prototype EDL933 (O157:H7) [35] (Table 1). The antigens Stx2 and Stx1 were commercially available and acquired from Phoenix Laboratory, Tufts Medical Center, Boston, MA, USA.
