*2.3. Expression of CD11a and CD11b on the Surface of Leukocytes Present in the Peritoneal Exudates from Mice Injected with Different Toxins*

The expression of the CD11a and CD11b adhesion molecules on the surface of leukocytes present in the mouse peritoneal exudates was investigated using flow cytometry 4 h after the injection of Jar, Jar-C or BnP1 and compared to the control group (Figures 3 and 4). Leukocytes from the PBS-treated group showed basal expression of CD11a (Figure 3A,E), and the number of cells expressing this molecule increased in the groups of mice injected with the three toxins (Figure 3B–E).

The expression of CD11b was not detected in peritoneal exudate cells from the control group. However, two other cell populations showed differences in the expression of the CD11b molecule, i.e., one population exhibited intermediate expression of CD11b and was designated CD11b+ cells, and the other exhibited high expression of this molecule and was designated CD11b++ cells (Figure 4A,E,F). In groups injected with the toxins, an increase in the CD11b+ population was observed compared to that observed in the exudate from the group injected with PBS (Figure 4A–E), but no differences in the CD11b++ population were observed (Figure 4F).

**Figure 3.** Expression of CD11a on the surface of leukocytes present in the peritoneal exudate of mice injected with different toxins. Peritoneal exudate cell suspensions were obtained within 4 h after the injection of PBS (**A**), Jar (**B**), Jar-C (**C**) or BnP1 (**D**) (2 μg/300 μL). Cells were incubated with anti-CD11a-FITC antibodies or isotype control-FITC. All incubations with anti-CD11a-FITC were performed on duplicate samples and analyzed using flow cytometry. Histograms are representative of one experiment. The bar graph shows the average numbers of CD11a-positive cells in each experimental group ± SD from three independent experiments (**E**). \* *p* < 0.05 compared to the PBS group.

**Figure 4.** Expression of CD11b on the surface of leukocytes present in the peritoneal exudate of mice injected with different toxins. Peritoneal exudate cell suspensions were obtained within 4 h after the injection of PBS (**A**), Jar (**B**), Jar-C (**C**) or BnP1 (**D**) (2 μg/300 μL). Cells were incubated with anti-CD11b-FITC antibodies or isotype control-FITC. All incubations with anti-CD11b-FITC were performed on duplicate samples and analyzed using flow cytometry. Histograms are representative of one experiment. The bar graph shows the average numbers of positively stained cells designated as CD11b+ (**E**) or CD11b++ (**F**) in each experimental group ± SD from three independent experiments. \* *p* < 0.05 compared to the PBS group.
