*5.8. Seizure Threshold*

At 60 days of age, one male and one female from each litter received an initial dose of 20 mg/kg PTZ, and a booster of 10 mg/kg every 10 min until the occurrence of score 5 or more according to the score scale adapted from that proposed by [50] (Table 1).

**Table 1.** Score scale adapted from that proposed by Fischer and Kittner (1998).


The total injected volume of PTZ varied approximately between 0.6 mL (initial dose plus a booster) and 1.2 mL (initial dose plus three boosters).

After the observation, the average score in each experimental group was computed. Statistical analyses were performed by the Kruskal–Wallis test, and the level of significance was set at *p* < 0.001 and *p* < 0.05.

**Author Contributions:** M.d.O.R.B. and A.L.A.N. conceived and designed the experiments; M.d.O.R.B. and M.E.F.d.V.d.P. performed the experiments and analyzed the data; M.d.O.R.B. and A.L.A.N. wrote the paper. All authors have read and agreed to the published version of the manuscript.

**Funding:** M.d.O.R.B. was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil).

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Institutional Review Board (or Ethics Committee) of Butantan Institute (Animal Care and Use Protocol 1927030818, approved 19 December 2018).

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

#### **References**

