*5.9. Data Analysis*

The data are presented as mean ± SD. ANOVA was used to calculate differences between groups and Tukey's multiple comparisons test was used as a posteriori. All Statistical analysis was performed using Graph Pad Prism Software 5.0 (San Diego, CA, USA).

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/ 10.3390/toxins13110825/s1, Figure S1: Binding of Fab:F8 to Stx2 measured by Biacore.

**Author Contributions:** Conceptualization, D.L., M.M.A. and R.M.F.P.; Formal analysis, D.L., F.D.G., R.L.F., B.S.M., B.E.C.G., W.Q., F.S. and G.C.; Funding acquisition, D.L., C.I., S.S.S., M.M.A. and R.M.F.P., S.S.S.; Investigation, D.L., F.D.G. and F.S.; Methodology, D.L., F.D.G., R.L.F., B.S.M., B.E.C.G., W.Q., A.P., F.S., G.C. and M.M.A.; Project administration, M.M.A. and R.M.F.P.; Resources, D.L., A.M.M., C.I., S.S.S., M.M.A. and R.M.F.P.; Supervision, C.I., S.S.S., M.M.A. and R.M.F.P.; Validation, D.L.; Visualization, F.D.G., R.L.F., B.S.M., B.E.C.G., W.Q., A.M.M., A.P., F.S., C.I., G.C. and S.S.S.; writing—original draft, D.L., M.M.A. and R.M.F.P.; Writing—review and editing, D.L., F.D.G., B.E.C.G., W.Q., F.S., C.I., G.C., S.S.S., M.M.A. and R.M.F.P. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by São Paulo Research Foundation (FAPESP–2015/17178-2;— 2018/13895-0 to R.M.F.P and 2013/03160-9, 2019/24276-1 to D.L) and by the "National Agency for Promotion of Science and Technology" (grant number ANPCYT-PICT 2017-0617 to María Marta Amaral), the "University of Buenos Aires" (grant number UBACYT-20020170200154BA to María Marta Amaral and UBACYT-20020170100600BA to Cristina Ibarra) and the "National Scientific and Technical Research Council" grant number CONICET: PUE 0041 to María Marta Amaral and Cristina Ibarra). RLF, an undergraduate student was a recipient of a fellowship from FAPESP (2018/24659-5) and Fundação Butantan, and currently, she is the recipient of a fellowship from the Brazilian National Council (PIBIC-CNPq). RMFP and AMM received a fellowship from National Council of Scientific and Technological Development (CNPq 303969/2017-2 and 307045/2020-0, respectively).

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Human Ethics Committee of the University of Buenos Aires (18 August 2018) and by the Bioethics Committee of National Hospital "Alejandro Posadas", Buenos Aires, Argentina (no: 035 LUP1S0/19 (13), 10 June 2019).

**Informed Consent Statement:** Human glomerular endothelial cells (HGEC) were isolated from kidney fragments removed from normal areas from different pediatric patients with segmental uropathies or tumors in one pole and normal creatinine that were undergoing nephrectomies performed at National Hospital "Alejandro Posadas", Buenos Aires, Argentina. Written informed consent was obtained from the next of kin, caretakers, or guardians on the behalf of the minors/children participants involved in our study.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding authors. The data are not available in the repository of the Butantan Institute (https://repositorio.butantan.gov.br (accessed on 16 November 2021)).

**Conflicts of Interest:** The authors declare no conflict of interest.
