*4.3. In Silico Analysis of Chitin Degrading Genes and Secondary Metabolites*

The assembled genome was analyzed using the CLC Main Workbench 8.0.1 (CLC bio, Aarhus, Denmark) and the online platform MaGe MicroScope [45]. The chitinolytic enzymes and the prediction of the chitin degradation pathway in S4059 were annotated according to the prediction of the S4059 proteome in Uniprot (UniProt ID UP000305729). Amino acid sequences of the candidate chitinases were also submitted to the SignalP 5.0 Server [46] to identify the signal peptides. In addition, genomes were submitted to antiSMASH version 6.0 (https://antismash.secondarymetabolites.org/#!/start (accessed on 15 January 2021)) for the prediction of putative biosynthetic gene clusters involved in the production of secondary metabolites.
