*4.12. Extraction of Metabolites for Chemical Analysis*

Bacterial cultures were grown in MMM with different carbon sources, and samples were taken for chemical analyses after 72 h incubation. Ten milliliters of the sample were extracted with an equal volume of high-performance liquid chromatography (HPLC)-grade Ethyl acetate in 50 mL Falcon tubes. The organic phase was transferred to a new vial and evaporated under nitrogen. The dried extract was re-dissolved in 500 μL methanol and stored at −20 ◦C. Chemical analysis was performed in biological triplicate.
