*3.6. Compound Isolation*

*M. bouillonii* biomass from Laulau Bay, Saipan, was thoroughly extracted with 2:1 dichloromethane and methanol, yielding 10 g crude extract from 132 g (1 L) biomass. A portion of the crude extract was fractionated over silica with vacuum liquid chromatography and a standardized solvent system protocol (Table S12). Two relatively polar fractions (fractions F and G) were found to contain the bulk of compound **1**. Reverse phase HPLC was used to isolate 2.6 mg of this compound from these two fractions. A gradient method from 37% to 50% CH3CN + 0.1% formic acid in H2O + 0.1% formic acid over 60 min at a flow rate of 4 mL/min resulted in the elution of compound **1** starting at a retention time of approximately 38 min.
