*3.2. Detection of Homodolastatin 16, Dolastatin 16, and Antanapeptin A in Kenyan Isolates of a Filamentous Cyanobacterium, Moorea producens*

The detection of homodolastatin 16, dolastatin 16, and antanapeptin A in *M. producens* was confirmed by LCMS analysis of organic extracts of the cyanobacterium. Briefly, *M. producens* (previously known as *L. majuscula*) (2 g) was extracted with 2:1 dichloromethane/ methanol (50 mL, 12 h, 25 ◦C, sonicated) and filtered through glass fiber filters (44 μ). Fractionation of the extracts from the Kenyan "*L. majuscula*" was achieved on C-18 silica to give "TD-Lyng chl" (the first Lyngbya extract fraction to elute from the C-18 column) and "TD-Lyngbia" that eluted immediately after "TD-Lyng chl". TD-Lyng chl and TD-Lyngbia were respectively evaporated and dried under nitrogen. The dried extracts were redissolved in 1:1 methanol/water to a final concentration of 2 mg/mL. Subsequently, 5 μL of the sample was injected onto the column (XBridge C18, 3.5 μm, 2.1 × 150 mm) and separated on a gradient of 10% 0.1% formic acid in water/90% 0.1% formic acid in acetonitrile to 5% 0.1% formic acid in water/95% formic acid in acetonitrile at 0.3 mL/min. Molecular ions for the cyclodepsipeptides were identified by Fourier transform mass spectrometry (FTMS) from ESI Full MS chromatograms. The MS used was a Thermo

Scientific Orbitrap Exactive (THERMO FISHER SCIENTIFIC, Bremen GmbH, Germany); resolution was set to 150,000.
