*4.10. Growth Curves of Wild Type and Mutant*

Growth kinetics of *P. rubra* S4059 WT (wild type) and Δ*GH19* (GH19 chitinase mutant) were established in MMM with or without 0.3% casamino acids and supplemented with 0.2% colloidal chitin, 0.2% crab chitin (C9752, Sigma, St. Louis, MO, USA), 0.2% mannose (63580, Sigma, St. Louis, MO, USA), or 0.2% NAG (A3286, Sigma, St. Louis, MO, USA), respectively, as carbon source. Pre-cultures of *P. rubra* WT and mutants were grown in MB overnight at 25 ◦C. The cultures were diluted to a starting concentration of 103 CFU/mL in MMM. Samples were taken every 4 h for estimation of cell density by plate counting. All experiments were done in three biological replicates.
