*2.9. Scanning Electron Microscopy (SEM)*

The adhesion and biofilm formation over the cotton fabric were assessed by SEM. The tubes containing MPB and specimens of untreated and treated cotton fabrics were inoculated with a suspension of *P. aeruginosa.* After incubation for 24 h, the specimens were washed with phosphate-buffered saline, dried and coated with gold with Jeol JFC-1200 fine coater (Jeol Ltd., Tokyo, Japan) and then investigated by Jeol JSM-5510 SEM (Jeol Ltd., Tokyo, Japan).
