2.3.1. Adhesion Test

Adhesion assay was performed 1.5, 2, and 4 h after cell seeding. Samples were washed with PBS prior to MTS reagent, added to rinse unattached cells. Next, 80 μL MTS reagent and 400 μL of fresh cell culture medium were added to each sample and reference. It was incubated for 4 h at 37 ◦C, H = 90% and a CO2 concentration of 5%. After that time, 100 μL of the reaction solution from every sample was transferred to a 96-well plate in triplicates. The absorbance at a wavelength of 490 nm was measured using a Microplate Reader (LT-4000, Labtech, Aylesbury, UK).
