*2.2. Synthesis of Acridine Dendrimer (ACR)*

A two-neckle round bottom flask, provided with a reflux cooler, magnetic stirring and N2 atmosphere was loaded with 0.5 g of 9-chloroacridine (2.3 mmol) and 1.15 g of distilled phenol. The reaction was maintained refluxing at 110 ◦C for 1 h. Then, 182.8 L of dendrimer (0.181 g, 0.23 mmol) was added, and reflux at 110 ◦C was maintained for 6 h. The reaction was then allowed to cool at room temperature, and 30 mL of acetone were added, under stirring, to the brown slurry obtained. The slurry solidified, and the yellow-orange solid obtained was washed with acetone. The solid was dissolved in water/NH4OH (pH 8) and extracted with dichloromethane (4 × 20 mL). The organic extracts were dried over anhydrous MgSO4 and evaporated under vacuum. 0.25 g (yield = 52%) of a yellow-orange solid were obtained. Tdecomp: 254 ◦C

FT-IR cm<sup>−</sup>1: 3240, 1615, 1594, 1558, 1518, 1466, 1138, 1020, 752, 647.

1H-RMN (CDCl3) ppm: 1.22 (s, 4H15, C-CH2-CH2-C), 1.57 (bs, 16H9 and 8H12, C-CH2-C ), 1.91–2.07 (bs, 8H11, 8H13 and 4H14, N-CH2), 2.28 (bs, 16H10, N-CH2), 3.60 (bs, 16H8, NH-CH2-), 7.03 (bs, 16H2, Ar-H), 7.43 (bs, 16H3, Ar-H), 7.89 (sa, 16H4 and 16H1, Ar-H).

13C-NMR (CDCl3) ppm: 23, 25, 29, 45, 51, 55, 115, 117, 121, 126, 129, 133, 141, 152.

API-ES-MS (positive) *m*/*z*: found: 2191.6 (100, [M + H]+), 1096.1 (65, [M + H]+/2).
