*2.9. Quantification of Loaded Compound in BC*

Lyophilised unloaded BC was aseptically cut into 8 mm disks and weighed. Then, the disks were individually placed into 1 mL aliquots of 50 mg/L (*w/v*) solutions of either thymoquinone, ocimene, miramistin, or amphotericin B for 24 h at room temperature under agitation (100 RPM). The concentration of 50 mg/L was chosen for all compounds, as it is twice the concentration of the highest MIC/MFC value and in accordance with standardised operating protocols [46,47]. After loading, the samples of BC were removed from the solutions and were again lyophilised by freezing at −20 ◦C for 24 h and lyophilised for a further 72 h and weighed again. All experiments were conducted ten times.
