*2.7. Minimum Inhibition Concentration (MIC)*

The MIC90 (where the antifungal drug inhibits the growth of 90% of the organism) of thymoquinone, ocimene, miramistin, and amphotericin B was determined for all fungal strains using a standardised methods: CLSI M27 and M38 [46,47]. Briefly, RPMI without bicarbonate was supplemented with 3-(N-morpholino)propanesulfonic acid (MOPS), 2% (*w*/*v*) glucose and pH adjusted to 7 with 10 M NaOH. All fungal strains were grown on SDA, and suspensions were made in the RPMI-1640 to 0.5 McFarland standard at 530 nm, approximately 5 × 106 CFU/mL. The microtiter plates were incubated for 24 h at 35 ◦C, and the OD570 was measured with a microtiter plate reader (SPECTROstar® Nano, BMG Labtech, Ortenberg, Germany). All experiments were repeated in triplicate.
