*2.3. Peptide Characterisation*

### 2.3.1. High-Pressure Liquid Chromatography

All peptides were purified with preparative HPLC using a Thermo Scientific Dionex Summit preparative HPLC system (Thermo Fisher Scientific Ltd., Loughborough, UK). Their purity was tested with a Thermo Scientific Dionex Summit analytical HPLC system. Both systems were equipped with C18 columns, water with 0.05% TFA was used as the hydrophilic medium, and acetonitrile with 0.05% TFA was used as the hydrophobic medium. The detection of molecules was conducted by measuring the UV absorbance of the amide bond at a wavelength of 210 nm. For preparative HPLC, a total of 2 mL of crude peptide dissolved in a mixture of acetonitrile and water (*v*/*v* = 1) was injected into the C18 column, while for analytical HPLC 100 μL of a similar peptide solution was added to the C18 column. In both cases, the columns were run for 60 min with a linear gradient of solvents starting at 100% dH2O and 0% acetonitrile, finishing at 0% water and 100% acetonitrile.
