2.2.6. Statistical Analysis

The statistical data on the WPI-TA hydrogels' swelling under conditions with different pH, both with and without TA, the TA release and the cytotoxic activity of the hydrogels were calculated using Microsoft Excel. Means and standard deviations were obtained from 3–6 independent experiments.

The data on the kinetics of swelling of hydrogels loaded with TA incubated in PBS at different pH values were plotted as "mean ± standard error" (*n* = 6). The viability of Hep2 cells incubated for 24 and 48 h with hydrogels containing different TA/WPI ratio was presented as "mean ± standard error" (*n* = 4). Differences between treatments were analyzed using two-way analysis of variance (ANOVA, Microsoft Excel 2016). [26] Calculations were carried out using Microsoft Excel software. Values of *p* ≤ 0.05 were considered significant (Tables S1–S4).
