*2.10. Zone of Inhibition (ZOI)*

Disk diffusion assays were conducted using a modified Kirby–Bauer procedure [48] following internationally recognised standardised techniques in CLSI M44 and M51 protocols [49,50]. Briefly, Mueller–Hinton (2% (*w/v*) glucose) agar plates were flooded with overnight broth cultures (0.5 McFarland) of either *C. auris*, *C. albicans*, *A. fumigatus,* or *A. niger*. Control plates of Mueller–Hinton (2% glucose) agar flooded with filter sterile RPMI-1640. Once the plates were inoculated, four 8 mm BC disks which were loaded with varying concentrations of the antifungal drug, were placed onto the surface of the seeded plates in triplicate and were incubated for 24 h at 35 ◦C. Pure BC discs loaded with water were used as controls. After incubation, the diameter of the clear zone (ZOI), including the diameter of the 8 mm disk, was measured and statistically analysed using one-way ANOVA post hoc Tukey (*p* < 0.05) (GraphPad Prism V. 9.0.1(151), GraphPad Software, San Diego, CA, USA).
