*2.13. Fourier Transform Infrared Spectroscopy*

Purified BC and BC loaded with the respective antifungal drugs were cut into 8 mm disks and again lyophilised and then analysed using a Bruker ATR FTRI spectroscope after running background scans (Bruker, Billerica, MA, USA). Each loaded BC sample was analysed in triplicate for each respective drug and was scanned 12 times at 400–4000 cm−<sup>1</sup> at room temperature.

## *2.14. Statistical Analysis*

All experiments in this project were conducted in triplicate, and all data presented are means± standard deviation (SD). Data recorded during all experiments were analysed statistically by one-way ANOVA post hoc Tukey (GraphPad Prism V. 9.0.1(151), GraphPad Software, San Diego, CA, USA) with (*p* < 0.05) for ZOI, MIC and MFC. *p* values for cytotoxicity studies were (*p* < 0.01).
