*2.5. GC-MS*/*MS Analysis*

The analysis was performed on a Shimadzu GC-MS TQ8040 equipped with Phenomenex ZB-Semivolatiles columns (30 m, 0.25 mm ID, 0.25 μm) and a tandem mass spectrometry detector. A sample volume of 1 μl was injected in the splitless mode at an injector temperature of 270 ◦C. The oven temperature was programmed as follows: initial temperature 60 ◦C (held for 2 min) increased by 70 ◦C/min to 200 ◦C; increased by 6 ◦C/min to 300 ◦C (held for 2 min). The ion source and interface temperature were held at 230 ◦C and 280 ◦C, respectively. The total analysis run time was 23 min. Quantitative and qualitative analyses were performed with LabSolution Insight software based on the two most intensive fragment ion transitions. The matrix matched standards were used for calibration and quantification, prepared by analyzing blank samples spiked with pesticides after the extraction and purification steps.
