Colocalization of Dopamine Transporter and Adenosine A2A Receptors

Chronic treatment with caffeine is proposed to operate through A2AR and was shown to affect DAT density and function. Pandolfo et al. [51] proved the colocalization of A2AR and DAT in striatal and frontocortical nerve terminals. The number of animals analyzed was three in the WKY control group, four in the WKY caffeine-treated group, three in the SHR control group and three in the SHR caffeine-treated group. In the striatum, statistical analysis revealed a significant effect of strain on the colocalization of A2AR and DAT immunoreactivities, and a subsequent comparison exhibited that nerve terminals from vehicle-treated SHR displayed a significantly lower colocalization of A2AR and DAT in comparison with vehicle-treated WKY. In the frontal cortex, a statistical analysis revealed no significant effect of strain or treatment on the colocalization between A2AR and DAT.
