*2.3. Scoring Severity of IMQ-Induced Skin Inflammation and Healing Assesment*

The severity of IMQ-induced skin inflammation and the progress of the disease, were daily evaluated using three in vivo parameters-erythema, thickening and skin scaling. These parameters were scored daily on a 0–4 scale such as 0—no change, 1—mild change, 2—marked change; 3—significant change, 4—severe change. By summing erythema, thickening and skin scaling daily scores, a modified PASI score was calculated (0–12 scale).

The body weight of the animals was monitored during the experiments (Scientech SL 3100D, Boulder, CO, USA). For the mice from IgY-treated Ps group the body weight was recorded at the beginning of the experiment (day 1), day 7 (beginning of IgY treatment), at the end of IgY treatment (day 12) and before being humanely euthanized—day 20 when it was macroscopically appreciated that the induced psoriasis disappeared. The mice from naturally remitted Ps group were allowed to heal naturally and weighed at the beginning of the experiment (day 1), after IMQ-based cream application (day 7) and before euthanization—day 22—when it was macroscopically appreciated that the effects

produced by IMQ-based cream disappeared. The mice from Ps group were weighed at the beginning of the experiment (day 1) and before sacrifice (day 7).
