4.3.4. LC-MS Analysis

The optimization of settings for LC and MS/MS detection was left to the participants. Examples of suitable LC-MS/MS settings were provided (they are reported in Figure 1A,B). Mandatory requirements were (i) the chromatographic separation of the two isomers 15-AcDON and 3-AcDON; (ii) using a tandem mass spectrometer (MS/MS), performing Selected Reaction Monitoring (SRM) in the case of MS/MS analyzers or Parallel Reaction Monitoring (PRM) in the case of MS/high-resolution mass spectrometry (HRMS) analyzers. For mycotoxin identification, it was requested to fulfill the criteria defined in the SANTE/12089/2016 document [32].
