*4.8. Annexin V-FITC/PI for Apoptotic Cells*

Treated cells were washed three times using PBS, harvested, and then resuspended with Annexin V-FITC and PI in binding buffer solution followed by 15 min incubation at room temperature. Apoptotic cells were then analyzed by a flow cytometry (FACSCalibur system, BD Biosciences, San Jose, CA, USA) using an Apoptosis Detection Kit (BD Pharmingen™, San Jose, CA, USA).
