*4.9. Hoechst 33342 Staining Analysis*

Treated cells were then washed, harvested, and then fixated using ethanol. After incubation for 20 min, 10 μM Hoechst 33342 staining was added following another 20 min incubation in room temperature. Observation was perform using a fluorescence microscope (Leica DMI6000 B, Wetzlar, Germany).
