*Article* **The Potential Protective Effect and Possible Mechanism of Peptides from Oyster (***Crassostrea hongkongensis***) Hydrolysate on Triptolide-Induced Testis Injury in Male Mice**

**Xueyan Zhang 1, Zhilan Peng 1, Huina Zheng 1,2,3,4,5,6, Chaohua Zhang 1,2,3,4,5,6, Haisheng Lin 1,2,3,4,5,6 and Xiaoming Qin 1,2,3,4,5,6,\***


**Abstract:** Peptides from oyster hydrolysate (OPs) have a variety of biological activities. However, its protective effect and exact mechanism on testicular injury remain poorly understood. This study aimed to evaluate the protective effect of OPs on triptolide (TP)-induced testis damage and spermatogenesis dysfunction and investigate its underlying mechanism. In this work, the TP-induced testis injury model was created while OPs were gavaged in mice for 4 weeks. The results showed that OPs significantly improved the sperm count and motility of mice, and alleviated the seminiferous tubule injury. Further study showed that OPs decreased malonaldehyde (MDA) level and increased antioxidant enzyme (SOD and GPH-Px) activities, attenuating oxidative stress and thereby reducing the number of apoptotic cells in the testis. In addition, OPs improved the activities of enzymes (LDH, ALP and ACP) related to energy metabolism in the testis and restored the serum hormone level of mice to normal. Furthermore, OPs promoted the expression of Nrf2 protein, and then increased the expression of antioxidant enzyme regulatory protein (HO-1 and NQO1) in the testis. OPs inhibited JNK phosphorylation and Bcl-2/Bax-mediated apoptosis. In conclusion, OPs have a protective effect on testicular injury and spermatogenesis disorders caused by TP, suggesting the potential protection of OPs on male reproduction.

**Keywords:** oyster peptides; spermatogenesis; oxidative stress; apoptosis; hormone; testis
