*4.6. Phenotype Assay*

DCs were harvested and washed twice with PBS, and incubated with FITC-MHCII, PE-CD40, PE-CD80, FITC-CD86, or their respective isotypes, at 4 ◦C for 30 min as per the manufacturer's guidelines. After being washed three times with PBS, DCs were analyzed by FCM.

#### *4.7. Endocytosis Assay*

The harvested DCs were incubated with 1 mg/mL FITC-Dextran at 37 ◦C for 30 min as previously described [54]. After incubation, DCs were washed twice with PBS and analyzed by FCM. In addition, 4 ◦C control was also performed to exclude adhesion.

#### *4.8. Migration Assay*

The chemotaxis of DCs was performed in a 24-well transwell chamber (pore size, 5 μm; Corning) as described previously [55]. DCs (1 × 105 cells) were then seeded onto the upper chambers and CCL19 (200 ng/mL) was added in the lower chamber. After incubation for 4 h, the migrated cells were collected from the lower chamber, and the number of cells was counted by FCM.
