2.2.1. Ethylene Emission

Ethylene emission was analyzed by placing five tomato fruits in 600 mL airtight glass containers at 25 ◦C for 1 h. Five 1 mL samples of gas produced in the headspace were then collected with gastight syringes through a rubber septum. A gas chromatograph with a flame ionization detector (Flame Ionization Detector (FID) for GC; (Agilent Technologies, Santa Clara, CA, USA, model HP-6890) and HP-PLOT Q column (30 m × 0.53 mm × 40 μm) were used to evaluate ethylene emission. The injector and detector temperatures were equally established at 250 ◦C and the oven at 30 ◦C. The helium gas flow was set at 1 mL.min−1, and the injections were performed using a pulsed splitless method.
