*2.2. Sample Preparation*

Each replicate sample of three fruits was blended with a homogenizer (Model VM0101, Vita-mix Corp., Cleveland, OH, USA) for one minute. The homogenate, 4.3 g, was pipetted into a 20 mL vial, mixed with 1.7 mL of a saturated CaCl2 solution [24], and crimp-capped with a silicone septum (Gerstel Inc., Linthicum, MD, USA). Samples were vortexed, flash frozen in liquid nitrogen, and stored at −80 ◦C until analysis.
