*2.8. Statistical Analysis*

Data obtained during shelf-life were subjected to mixed ANOVA, applying SPSS ver.21 (SPSS, Chicago, IL, USA) at *p* < 0.05. Data from the first experiment were analysed by mixed ANOVA with oxygen level and maturity as between subject factors and days in storage as within subject factor. For the second experiment, mixed ANOVA was carried out with oxygen level and FR as between subject factor and days in storage as within subject factor. Normality of the variables was tested applying the Shapiro-Wilk test. Mauchly's test of sphericity was carried out to test whether variances of the differences between all possible pairs of within-subject conditions were equal. If the sphericity assumption was not fulfilled, Greenhouse-Geisser's correction was applied to calculate the degrees of freedom. In case of a significant interaction, a pairwise comparison was carried out for each shelf-life day with LSD (Least Significant Difference) values estimated.
