KEGG Functional Annotation

The enriched KEGG pathways could be divided into five categories, including "cellular processes", "environmental information processing", "genetic information processing", "metabolism", and "organic systems". According to the KEGG pathway classification (Figure S2A,C) and enrichment analysis (Figure S2B,D), the DEGs were mainly associated with metabolism pathways. As shown in Table S7, the C0 vs. C14 and C0 vs. CS14 comparisons indicated that "plant hormone signal transduction", "protein processing in endoplasmic reticulum", "ribosome", "phenylpropanoid biosynthesis", "starch and sucrose metabolism", "biosynthesis of amino acids", and "carbon metabolism" were the main metabolic pathways affected by different low-temperature storage conditions. Among them, the "phenylpropanoid biosynthesis", "starch and sucrose metabolism", and "biosynthesis of amino acids" pathways are associated with the synthesis of partial free phenolic acids, sugars, and free amino acids, respectively. The present study mainly focused on these three metabolic pathways by screening key genes linked to metabolic changes.
