*3.3. Experiment 2: Effects of Low Oxygen Storage of Mature Green Tomatoes Cultivated with and without Far Red Lighting on CI Symptoms, Weight- and Firmness Loss*

 ι ι Tomatoes cultivated without far red lighting during cultivation showed CI symptoms during shelf-life. The lowest pitting index was observed for MG tomatoes stored at 1 kPa O2, the highest for the low oxygen control (Figure 5A). MG tomatoes cultivated with far-red lighting demonstrated reduced CI compared with tomatoes grown without FR lighting. In fact, no CI symptoms were observed for all low oxygen treatments, even after 3 weeks of shelf-life (Figure 5B). There were no chilling symptoms in fruit stored at 12 ◦C, and no differences were observed in terms of weight loss (Figure S2).

**Figure 5.** Chilling injury symptoms as indicated by the disorder index of MG tomatoes cultivated under white LED light (WL) without far red lighting (**A**) or with far red lighting during cultivation (**B**) during storage (blue area) at 2 or 12 ◦C and shelf-life at 20 ◦C (white area). Orange, green, red and purple lines and symbols indicate 1, 5 and 21 kPa O2 (low oxygen control) applied during storage at 2 ◦C, and 21 kPa O2 at 12 ◦C (temperature control), respectively. The average disorder index with indicated standard error is shown for two replicates of four tomatoes (*n* = 2); (repeated measure over times). LSD values (*p* < 0.05) are indicated per panel.

Firmness at harvest was similar for MG tomatoes cultivated with or without FR lighting (*p* > 0.05). Softening during storage at 2 ◦C for was faster for MG tomatoes that were cultivated without- compared to those with FR lighting (*p*< 0.05) (Figure 6). Softening of tomatoes cultivated without FR was similar during storage and shelf-life independent of

the storage oxygen concentration. Tomatoes cultivated without FR from the temperature control treatment showed no softening during storage (Figure 6A), but tomatoes cultivated with FR showed similar softening for all treatments (Figure 6B).

**Figure 6.** Firmness as indicated by firmness index (FI) of MG tomatoes cultivated under white LED light (WL) without far red lighting (**A**) or with far red lighting during cultivation (**B**) during cold storage (blue area) at 2 or 12 ◦C and subsequent shelf-life at 20 ◦C (white area). Orange, green, red and purple lines and symbols indicate 1, 5 and 21 kPa O2 (low oxygen control) applied during storage at 2 ◦C, and 21 kPa O2 at 12 ◦C (temperature control), respectively. The average firmness index with indicated standard error is shown for two replicates of four tomatoes (*n* = 2); (repeated measure over times). LSD values (*p*< 0.05) are indicated per panel.
