**5. Conclusions**

Application of farmyard manure three years before wheat was sown significantly a ffected wheat grain yields in all localities and positively influenced soil chemical properties, but has no further beneficial e ffect on grain quality.

Application of mineral nitrogen significantly increased grain yield and grain quality in all localities, and also positively a ffected Cox and Nt concentration. No e ffect on the pH value was recorded in Chernozem and Cambisol soil types, but Phaeozem reacted on N fertilizers with a decrease of the pH value.

Application of mineral nitrogen, phosphorus, and potassium was not connected with any significant increase in grain yields when compared with application of only mineral nitrogen. On the other hand, soil P and K concentrations were statistically significantly higher in treatments with P and K, and their application prevents depletion of these elements in the soil.

The highest yields were recorded between 70 and 98 kg N ha−<sup>1</sup> in Ivanovice, 55 and 72 kg N ha−<sup>1</sup> in Caslav, and with 155 kg N ha−<sup>1</sup> in Lukavec.

Grain quality is mainly a ffected by nitrogen supply and limited by natural soil–climate barriers of the locality.

**Author Contributions:** Conceptualization, L.H., L.M., and E.K.; methodology, L.H., L.M., and E.K.; software, L.H.; validation, L.H. and L.M.; formal analysis, L.H.; investigation, L.H.; resources, E.K.; data curation, L.H.; writing—original draft preparation, L.H.; writing—review and editing, L.H.; visualization, L.H.; supervision, E.K.; project administration, E.K.; funding acquisition, E.K. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Ministry of Agriculture of the Czech Republic via the project RO 0418.

**Acknowledgments:** We would like to thank the laboratory sta ff, technicians, and all their predecessors for their work on long-term fertilizer experiments.

**Conflicts of Interest:** The authors declare no conflict of interest.
