*2.3. Quality Measurements*


#### Index = F/RS

#### *2.4. Measurement of Antioxidant Capacity*

Antioxidant capacity of methanol extracts was determined by means of spectrophotometric method using a cationic radical (ABTS+) [2,2 -azinobis-(3-ethylbenzothiazoline-6 sulfonic acid)] [32]. The ABTS+ cation was generated by mixing 7 mM ABTS and 2.45 mM K2S2O8 (potassium persulfate or potassium peroxydisulfate) solutions at a ratio of 1:0.5. The ABTS+ cationic radical solution and 70% methanol extract (*v*/*v*) samples were diluted using Phosphate Buffer Solution (PBS) pH 7.4. Absorbance was measured at a wavelength of 734 nm on samples incubated for 6 min at 30 ◦C against PBS as a reference assay. Antioxidant capacity was determined based on the percentage reduction in absorbance of the ABTS+ cationic radical solution by the sample compared to the reducing power of Trolox (6-hydroxy-2.5.7.8-tetramethylylchromate-2-carboxylic acid). The measurements were conducted using a Helios Alpha spectrophotometer (Thermo Electron Corporation Waltham, MA, USA) equipped with a water bath for the thermostatting of samples. The results were expressed as an average of nine repetitions per sample and expressed in μmol Trolox/g d·m.
