**2. Materials and Methods**

#### *2.1. Animals*

Adult (200–250 g) male Lewis and BN rats were purchased from Envigo (Indiannapolis, IN, USA) and housed in the animal care facility at the University of Wisconsin in Madison, WI, USA. All procedures were performed in accordance with the Animal Care and Use Policies at the University of Wisconsin. Animal health maintenance, including animal deaths, room temperature, 12 h light/dark cycles, and cage cleaning, among other sanitation duties, were performed daily by animal care staff. Food and water were available ad libitum. This research was prospectively approved by School of Medicine and Public Health Institutional Animal Care and Use Committee at the University of Wisconsin (Animal Protocol #M005204). All groups contained 4–6 animals.
