*3.2. Extraction of EOs*

The air-dried powder of the above-ground parts of the *P. dioscoridis*, and *E. bonariensis*, (200 gm, each) were subjected separately to Clevenger-type apparatuses using round flask (2.5 L) comtaining water (1.5 L) for hydro-distillation for 3 h. The oily layer of each plant was isolated separately by *n*-hexane, then dried using anhydrous Na2SO4 (0.5 g), and finally stored in glass vials in the freezer till further analysis via GC-MS. This extraction of the EO of each plant was repeated as duplicates.
