4.1.1. Fungal Isolate and Tomato Variety

The studied *Fusarium oxysporum* isolate was investigated as an aggressive fungal pathogen in tomato and other plants and was deposited in GenBank under accession number (KJ831189) and obtained from the Department of Plant Protection and Biomolecular Diagnosis, Arid Lands Cultivation Research Institute, Alexandria (Egypt). The fungal isolate was maintained on potato dextrose agar (PDA) slants and stored at 4 ◦C until further bioassay. Tomato seed (super strain B) variety was obtained from the Egyptian Ministry of Agriculture.

#### 4.1.2. Medicinal Plant Materials

Leaves of *M. spicata* and *M. longifolia* were collected from unrestricted habitats, Alexandria (Egypt), in June 2020. The plants' identification was performed in the Botany Department, Faculty of Science, Mansoura University, Mansoura (Egypt).

#### *4.2. Extraction of Essential Oils*

Previously collected *M. spicata* and *M. longifolia* healthy fresh leaves at age one month were prewashed with tap water, disinfected with 2% sodium hypochlorite for 30 min, then rinsed with sterile distilled water. Air-dried plant leaves were homogenized to a fine powder in a mill, stored in airtight dark bottles, and kept until use. Leaf powders were subjected to hydrodistillation for 3 h using a Clevenger-type apparatus, Shiva Scientific Glass Pvt. Ltd., New Delhi, India [72].
