*4.12. Statistical Analyses*

All the experiments were performed in triplicates. Obtained data and results were expressed as mean ± standard deviation (±SD). Some experiments were arranged in a completely randomized blocks design and data were statistically analyzed by one-way ANOVA test using SPSS 16. The probability values *p* ≤ 0.05 were considered statistically significant based on Duncan's least significant difference test. The heatmap was constructed to study the similarity and dissimilarity among studied taxa based on essential and nonessential amino acids using the TBtools package [90].
