*3.4. Data Analysis*

The experiment of phytotoxicity was repeated three times with three replications. The data of the inhibition were subjected to one-way ANOVA, followed by Duncan's test using CoStat program (version 6.311, CoHort Software, Monterey, CA, USA), while the IC50 values were subjected to a two-tailed *t*-test using MS-EXCEL. To make a holistic categorization of the EOs of the two studied ecospecies (Saudi and Egyptian) and other reported ecospecies (Algerian, Egyptian, Iranian, Sudanian, and Yemeni), we constructed a data matrix of the 30 major chemical compounds, with concentration > 3%, from 11 ecospecies. The matrix was subjected to Principal component multivariate data analysis (PCA) and agglomerative hierarchical clustering (AHC) using the XLSTAT Statistical Software package (version 2018, Addinsoft Inc., New York, NY, USA).
