**5. Conclusions**

In the present study, EO was isolated from *Anisosciadium lanatum* using a hydrodistillation method and its components were identified using GC-MS. The yield of EO was 0.46% *v/w*. In total, 38 components were identified and quantified. The findings suggest that the EO from *A. lanatum* has a controlling effect on hepatoma cells (i.e., the HepG2 liver cancer cell line) through regulation of the apoptotic markers BCL-2 and CASPASE-3. Molecular docking analysis supported the experimental results, which revealed the oxygenated molecules camphor, eucalyptol, nerol, and linalool as having potential virtual binding against the anti-apoptotic marker BCL-2, whereas camphor and eucalyptol alone showed significant binding against the pro-apoptotic regulator CASPASE-3. The obtained results indicate that the EO extracted from *A. lanatum* should be further explored in vivo as a tool for the management of hepatoma cancer diseases.

**Author Contributions:** H.E.K., conceptualization, funding acquisition, methodology, writing review and editing, writing—original draft preparation, and supervision; H.-I.M.I., formal analysis, methodology, validation, software, writing—review and editing, and writing—original draft preparation; H.M.D., methodology, software, and writing—review and editing; and K.M., conceptualization, supervision, and writing—original draft preparation. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Deanship of Scientific Research (DSR), King Faisal University, Saudi Arabia, research group project (grant number 1811002).

**Acknowledgments:** The authors thank the Deanship of Scientific Research (DSR), King Faisal University, Saudi Arabia for funding this research group project (grant number 1811002) and the College of Clinical Pharmacy, King Faisal University.

**Conflicts of Interest:** The authors declare that there are no conflicts of interest.

#### **References**

