*2.3. Migration (Scratch Wound Assay)*

The suppressive effect of the EO on migration was evaluated by scratch wound assay. In the scratch mobility assay, HepG2 liver cancer cells were treated with 25 μg/mL of EO supplemented with 1% FBS. The scratch wound was observed to determine the mobility of the cells in the junction, and scratch distance was measured to determine the closure disturbance, compared with that of the untreated control cells. The vehicle-treated HepG2 liver cancer cells significantly migrated after 24 h, whereas a distinct gap remained in the EO-treated groups after 24 h. The gaps were significantly suppressed (0.79 ± 0.05) by EO treatment (Figure 3). To confirm the inhibition effects, a gelatin-coated transwell insert assay was performed for invasion analysis. The relative migration fold of the EO-treated HepG2 liver cancer cells revealed significant differences compared with the untreated cells.
