*2.5. EO Extraction by Hydrodistillation*

The EO isolation was done by hydrodistillation method using Clevenger type apparatus. The seasonal variation influence on flowers' EO yields were examined, in which three (3) replicates were considered.

The seasonal variations' influence on *A. javanica* flowers' EO was tested. The summary of the tested treatments (mentioned as points) is represented in Table 1.


**Table 1.** Summary of *A. javanica* experimental treatments.
