*4.4. Preparation of EOs*

The *M. spicata* and *M. longifolia* EOs colloid solutions were prepared by slowly adding 20 mL of *M. spicata* and *M. longifolia* EOs to 1 mL of non-ionic surfactant Tween 80 (1%), and the dispersion was performed under gentle stirring. Then, 80 mL of distilled water was added to reach the final mixture of 100% with continuous stirring using a magnetic stirrer for 30 min. The mixture was fed into a liquefied potato dextrose medium at different concentrations for further in vitro antifungal activity assay and greenhouse experiments.

#### *4.5. In Vitro Antifungal Activity of EOs*

Assessment of the antifungal activity of *M. spicata* and *M. longifolia* EOs were conducted in vitro and evaluated against *F. oxysporum* radial mycelial growth using the agar plate technique according to Tatsadjieu et al. [74]. The *M. spicata* and *M. longifolia* EOs were liquefied in sterilized PDA media to obtain a final concentration of 0.25%, 0.5%, 0.75%, 1.0% and 1.25%. Twenty mL of broth medium was poured into Petri dishes (90 mm diameter). Plates supplemented with 0.05% of fungicide (nystatin at 0.5 μL/mL) were used as control. Sterile distilled water was used in the bioassays instead of EO as a negative control. All plates were inoculated with mycelial disc (5 mm diameter) of *F. oxysporum* from the PDA plate margins (5–7 days old). Three replicate plates were used for each treatment. Then, the Petri-dishes were incubated at 25 ◦C and the fungal colony diameter was measured at 7 days.
