*4.2. Plant Material*

The leaves of *J. rugosa* were collected in February 2020 in the south-central area of the Ecuadorian highlands (sector Citar, province Cañar). The specimens grew at an altitude of 3445 m above sea level, with coordinates 02◦35 387" S and 78◦56 309" W. The collection was carried out under governmental permission (MAAE-ARSFC-2020-0638), issued by the Ministry of Environment of Ecuador (MAE). The identification was achieved at the Universidad Técnica Particular de Loja, by the botanist Dr. Itziar Arnelas, and a botanical specimen was deposited at the UTPL herbarium, with voucher number D-HUTPL-2020-6. The fresh leaves of *J. rugosa* were distilled the day after collection.
