*4.3. Distillation of the EO and GC Sample Preparation*

Fresh leaves (4.8 kg) of *J. rugosa* were steam distilled in a Clevenger-type apparatus for 4 h. The EO, which spontaneously separated from the aqueous layer, was immediately dried on anhydrous sodium sulphate and stored in the dark at −15 ◦C until use. The analytical samples for each GC injection were prepared as described in previous studies [18–22].
