**5. Conclusions**

We can conclude that EOs of two *Mentha* aromatic plants (*M. spicata* and *M. longifolia*) have the highest potential against Fusarium root rot disease for tomatoes. The chemical essential oils of these EOs have a lethal effect against *F. oxysporum* fungi. In addition, these EOs enhance the growth of tomato plants by increasing their physiological activities. Due to the pathogen attack, this activity could be induced by the plant defense system. This activity was regulated by different families of genes such as WRKY and PR proteins. On the other hand, *L. esculentum* seed priming or seedling root treatments with *M. spicata* and *M. longifolia* EOs could be used at lower concentrations (1.0–1.25%) to enhance seedling growth and alleviate the adverse effects of the fungal disease by supporting antioxidant enzymes and total phenols accumulation which could help make seeds or plants tolerate oxidative stress conditions further. Finally, the EOS of *Mentha* medicinal plants can be used as a safe alternative to fungicides that improve the growth of the infected plant without defects in plant and human health.

**Supplementary Materials:** The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/plants11020189/s1, Figure S1. Relative expression level of WRKY1 gene in *S. lycopersicum* seedling under *Fusarium* inoculation and application of *M. spicata* and *M. longifolia* EOs. Different letters indicate significant differences between different treatments at *p* ≤ 0.05. Figure S2. Relative expression level of WRKY4 gene in *S. lycopersicum* seedling under *Fusarium* inoculation and application of *M. spicata* and *M. longifolia* EOs. Different letters indicate significant differences between different treatments at *p* ≤ 0.05. Figure S3. Relative expression level of WRKY33 gene in *S. lycopersicum* seedling under *Fusarium* inoculation and application of *M. spicata* and *M. longifolia* EOs. Different letters indicate significant differences between different treatments at *p* ≤ 0.05. Figure S4. Relative expression level of WRKY53 gene in *S. lycopersicum* seedling under *Fusarium* inoculation and application of *M. spicata* and *M. longifolia* EOs. Different letters indicate significant differences between different treatments at *p* ≤ 0.05. Figure S5. Relative expression level of Chitinase gene in *S. lycopersicum* seedling under *Fusarium* inoculation and application of *M. spicata* and *M. longifolia* EOs. Different letters indicate significant differences between different treatments at *p* ≤ 0.05. Figure S6. Relative expression level of defensin gene in *S. lycopersicum* seedling under *Fusarium* inoculation and application of *M. spicata* and *M. longifolia* EOs. Different letters indicate significant differences between different treatments at *p* ≤ 0.05.

**Author Contributions:** Conceptualization, S.A.S., E.E.H. and E.-S.S.A.R. and A.A.I.; data curation, E.-S.S.A.R., S.S.A.K., A.A.I. and H.S.E.; formal analysis, E.E.H., S.A.-E. and A.A.I.; investigation, S.A.S.; methodology, E.-S.S.A.R., S.A.-E. and A.A.I.; resources, E.E.H., E.-S.S.A.R. and A.A.I.; software, A.M.G.A.-K. and E.-S.S.A.R.; supervision, E.E.H. and H.S.E.; validation, E.E.H., S.S.A.K. and H.S.E.; visualization, S.A.S., A.M.G.A.-K., E.-S.S.A.R., S.A.-E. and A.A.I.; and writing—review and editing, A.A.I. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Relevant data applicable to this research are within the paper.

**Conflicts of Interest:** The authors declare no conflict of interest.
