*4.9. mRNA Expression*

mRNA from treated cells was isolated using Trizol Reagent (Sigma, Darmstadt, Germany) and purified using chloroform and isopropanol. Synthesis of cDNA was carried out using an RT multiscreen kit and amplification was carried out using a thermocycler PCR machine (Eppendorf, Hamburg, Germany). Quantitative analysis of target mRNA such as BCL-2, CASPASE-3, CYP-1A1, and NFkB was performed using a SYBR green master mix (Bio-Rad, California, CA, USA). Details of the primers used for real-time PCR are given in Table 4. Quantification of mRNA expression was achieved using the quant studio software (Bio-Rad, California, CA, USA). Data were normalized to GAPDH levels. All qPCRs were performed at least in triplicate for each experiment [56].

**Table 4.** Real-time PCR primer details.


*4.10. Computational Docking Analysis*
