4.9.2. ABTS Radical Cation Scavenging Capacity

The ABTS assay was performed using 2,2 -azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS•+) according to the procedure report by Arnao et al. [44] and Thaipong et al. [43], as described by Valarezo et al. [33]. The concentrations of the essential oil from *A. cherimola* used were 1000, 500, 250, 100, 50 and 25 ppm. The samples were evaluated in a UV spectrophotometer (Genesys 10S UV.Vis Spectrophotometer, Thermo Scientific, Waltham, MA, USA) at a wavelength of 734 nm. Deionized water was used as a blank control and trolox was used as a positive control. The percentage of scavenging capacity was calculated according to equation.

$$\text{Sc}(\%) = \frac{(\text{ASO} - \text{ASA})}{\text{ASA}} \ast 100\tag{3}$$

where ASO is the absorbance of ABTS•<sup>+</sup> with solvent mixture and Ai is the absorbance after reaction of ABTS•<sup>+</sup> with the sample.
