*4.5. Migration (Scratch-Wound Assay)*

The HepG2 cells were seeded in 6-well plates with a cell population of <sup>1</sup> × <sup>10</sup> <sup>5</sup> cells/well in complete DMEM/F12 medium and allowed to attach overnight in a CO2 incubator. The media was aspirated with DMEM/F12 with 25% charcoal-stripped FCS for 24 h. After the cells attained confluence, a scratch line was made in the centers of the wells using a sterile tip. The wells were then gently washed with serum-free DMEM/F12 medium and treated again with either DMSO or EO containing DMEM/F12 medium for another 24 h [37]. The scratch-line recovery was recorded using an Optika inverted microscope (200× magnification).

#### *4.6. Invasion (Transwell Assay)*

The transwell invasion assay was evaluated using a matrigel-coated 12-well Boyden chamber (8 μm PET; Corning, NY, USA), following the methodology described previously by Hanieh et al. (2016) [46], where 4 × 104 cells were cultured in the upper chamber in 600 μL of serum-free DMEM medium with EO. In the lower chamber, 800 μL of DMEM medium with 10% FBS were added.
