3.5.1. Cell Culture

The three cancer cell lines, breast adenocarcinoma cells (MCF-7), lung cancer cells (A-549), and hepatocellular carcinoma cells (HepG2) were obtained from VACCERA, Mokatam, Giza, Egypt. Cells were maintained in DMEM media supplemented with 100 mg/mL of streptomycin, 100 units/mL of penicillin, and 10% of heat-inactivated fetal bovine serum in humidified, 5% (*v/v*) CO2 atmosphere at 37 ◦C.
