*4.4. Moisture Determination*

Method Loss on drying (Moisture) in plants, AOAC 930.04-1930, was used to determine the moisture of plant material, for this an analytical balance (Mettler AC 100, Mettler Toledo, Columbus, OH, USA) was used. Moisture was calculated according to Equation (1).

$$\text{Moisture} \left( \% \right) = \frac{\text{wi} - \text{wo}}{\text{wi}} \ast 100 \tag{1}$$

where wi is the initial weight of sample and wo is weight of sample after drying.
