*4.6. Determination of the Physical Properties of the Essential Oil*

Density of the essential oil was determined using the ISO 279:1998 standard (equivalent to the AFNOR NF T 75-111 standard). Density measurement was performed using an analytical balance (Mettler AC 100, Mettler Toledo, Columbus, OH, USA) and a pycnometer of 1 mL. Refractive index was determined using the standard ISO 280:1998 [37] (similary to AFNOR NF T 75-112), for which a refractometer (model ABBE, BOECO, Hamburg, Germany) was used. An automatic polarimeter (Mrc-P810, MRC, Holon, Israel) was used to measure the optical rotation of the EO according to the standard ISO 592:1998. All measurements were taken at 20 ◦C.
