*2.3. Enantioselective Evaluation of the EO*

The enantioselective analysis of the EO was carried out on a 2,3-diethyl-6-*tert*butyldimethylsilyl-β-cyclodextrin based capillary column. Only the very minor compound (1*S*,2*R*,6*R*,7*R*,8*R*)-(+)-*α*-copaene could be certainly identified, appearing enantiomerically pure in the EO. No more enantiomeric pairs or enantiomerically pure compounds could be identified in the enantioselective chromatogram.

#### *2.4. AChE Inhibition Activity*

The AChE inhibitory activity of the investigated essential oils was measured in vitro, using a spectrophotometric assay based on Elman's method. Galanthamine and *Laurus nobilis* EO were used as positive controls, the latter being considered an active EO in literature (see Section 3. Discussion). All results are summarised in Table 2.

**Table 2.** Percent inhibition of AChE by *J. rugosa* EO compared to *L. nobilis* EO and galantamine as positive controls.

