2.6.2. Standard Curves

The standard curves were prepared using Trolox as a strong antioxidant reagent. The absorbance of Trolox at different concentrations was measured at 517 nm, 593 nm, and 734 nm for DPPH, FRAP, and ABTS assays, respectively. The results of all conducted assays were calculated and expressed as mg Trolox equivalents/g EO extract. The standard curves are illustrated in Figures 1–3.

**Figure 1.** Standard curve of DPPH assay.

**Figure 2.** Standard curve of FRAP assay.

**Figure 3.** Standard curve of ABTS assay.

#### *2.7. Chromatographic Analysis*

EO sample analysis was performed on an Agilent gas chromatograph (GC) equipped with flame ionization detection (FID) and an HP-5MS capillary column, 30 m length × 0.25 mm internal diameter, 0.25 μm film thickness, and temperature programmed as follows: 70–300 ◦C at 10 ◦C/min. The carrier gas was helium at a flow of 3.0 mL/min; injector port and detector temperature were 250 ◦C and 300 ◦C, respectively. One microliter of the sample (diluted with acetone, 1:10 ratio) was injected by splitting and the split ratio was 1:100.
