*4.9. Antioxidant Capacity*

#### 4.9.1. DPPH Radical Scavenging Capacity

The DPPH assay was performed using 2,2-diphenyl-1-picrylhydryl free radical (DPPH•) based on the technique described by Brand Williams et al. [42] and Thaipong et al. [43] according to what was described by Valarezo et al. [33]. The concentrations of the EO from *A. cherimola leaves* used were 1000, 800, 600, 450, 300, 150 and 25 ppm. Trolox was used as a positive control and methanol as a blank control. The samples were evaluated in a UV spectrophotometer (Genesys 10S UV.Vis Spectrophotometer, Thermo Scientific, Waltham, MA, USA) at a wavelength of 515 nm. The percentage of scavenging capacity

was calculated according to Equation (2). SC50 is the EO concentration that provided 50% DPPH• scavenging effect.

$$\text{SC}(\%) = \frac{(\text{AEO} - \text{AMeOH})}{\text{AEO}} \ast 100 \tag{2}$$

where AEO is the absorbance of DPPH• mixed with EO and As is absorbance of DPPH mixed with methanol.
