*5.10. Cell Culture*

Chinese hamster ovary cells stably overexpressing human APP (CHO-APP) were a generous gift from Dr. Woojin Kim (Faculty of Medicine and Health, University of Sydney, Sydney, Australia). The cells were generated by transfecting CHO cells with recombinant vectors expressing human 695-amino acid APP cDNA and a puromycin resistance gene, as described [91]. Cells were routinely maintained in F-12 growth medium supplemented with heat-inactivated foetal bovine serum (FBS; 10% *v*/*v*), L-glutamine (2 mM), penicillin (100 units/mL) and streptomycin (100 µg/mL), with the addition of puromycin (7.5 µg/mL), at 37 ◦C in humidified air containing 5% CO2.

SK-N-SH human neuroblastoma cells were obtained from Sigma-Aldrich (Castle Hill, NSW, Australia) and maintained in MEM growth medium supplemented with heatinactivated FBS (10% *v/v*), L-glutamine (2 mM), penicillin (100 units/mL) and streptomycin (100 µg/mL) at 37 ◦C in 5% CO2.
