*5.16. Statistical Analysis*

Data were analysed using Graphpad Prism (v6.01, La Jolla, CA, USA) and Microsoft Excel 2019. Values are expressed as mean ± SEM. MTT and ELISA data were statistically analysed using one-way ANOVA followed by Dunnett's test. Two-tailed unpaired Student's *t* test was used to evaluate differences between WT and P-gp KO mice. A *p* value of 0.05 was considered significant (\* *p* ≤ 0.05, \*\* *p* ≤ 0.01, \*\*\* *p* ≤ 0.001).

**Author Contributions:** Conceptualization, A.B.C., R.C. and I.C.G.; methodology, all authors; software, A.B.C.; validation, all authors.; formal analysis, A.B.C., R.C., A.M.S.H. and I.C.G.; investigation, all authors; writing—original draft preparation, A.B.C.; writing—review and editing, all authors.; supervision, I.C.G. and R.C.; project administration, I.C.G. and R.C.; funding acquisition, I.C.G., A.M.S.H. and R.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by a seed grant from the University of Sydney, Australia. A.B.C. and A.Y. were recipients of Australian Government scholarships. A.M.S.H. was supported by grant number 2R01AG039621 from the National Institute on Aging. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute on Aging or the National Institutes of Health.

**Institutional Review Board Statement:** Animal experiments reported in this article were approved by the Institutional Animal Care and Use Committee of the University of Kentucky and carried out in accordance with AAALAC regulations, the US Department of Agriculture Animal Welfare Act, and the Guide for the Care and Use of Laboratory Animals of the NIH.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding authors.

**Conflicts of Interest:** The authors declare no conflict of interest.
