*4.1. Yeast*

In addition to its convenience for genomic modification, *Saccharomyces cerevisiae* is a particularly interesting model for studying the peroxisomal metabolism of lipids, since it can use FAs as its only carbon source and β-oxidation of FAs of all length takes place only in peroxisome. The yeast model expresses only two peroxisomal ABC transporters, called Pxa1p and Pxa2p, which function as a strict heterodimer to import fatty acyl-CoAs into the peroxisomal matrix [107–109]. Functional assays and functional complementation experiments of pxa1/pxa2∆ yeast mutants with mammalian peroxisomal ABC transporters were particularly important in studying their transport mechanism and substrate specificity [26,65,75].
