*4.12. Statistical Analysis*

Statistical analysis of data was carried out using GraphPad Prism 8.1 (San Diego, CA, USA). For multiple comparisons with one independent variable, a one-way ANOVA was used with Dunnett's post-hoc test. For multiple comparisons with two independent variables, a two-way ANOVA was used with Dunnett's post-hoc test. A value of *p* < 0.05 was considered significant.
