2.3.2. Q347A and Q990A Also Act Synergistically to Reduce the Transport of BODIPY-Verapamil

− − − The Q347A and Q990A mutants (normalized transport activity of 81 ± 8% and 105 ± 16%, respectively (Appendix A Table A2)) combine in the Q347/990A double mutant to reduce the transport of BODIPY-verapamil to 38 ± 10% of the wild-type level (Figure 4). This double mutant also retains the ability to efflux BODIPY-verapamil because it is significantly different to the Walker B mutant E556/1201Q.
