*4.2. Alignment Analyses*

Multi-sequence alignment analyses were performed with the help of Jalview software (Consortium Project; www.jalview.org/) [54]. Results of the two alignment algorithms–Clustal Omega (EMBL-EBI; https://www.ebi.ac.uk/Tools/msa/clustalo/) and Mafft (EMBL-EBI; https://www.ebi.ac.uk/Tools/msa/ mafft/)—were considered. Alignments of individual ABCA genes and orthologs as well as all ABCA genes together were calculated. Figures S1 and S2 show the results of the alignment analysis for the 5 ′UTRs of the human ABCA1 gene and its vertebrate orthologs with nucleotide percentage identity colored, consensus logos and occupancy score histograms. Figures S3 and S4 show a similar analysis where the 5′UTRs of all 12 human ABCA genes were aligned together. Based on these analyses and criteria, the most conserved subregions within the 5′ UTRs were described in relation to the sATGs. The cut-off values for the identity and occupancy scores were set to 80%.
