*2.16. Ribo-Seq Data Confirmed Translation at Some of the Predicted uORFs*

The analysis of the Ribo-seq coverage data confirmed a high concentration of ribosomes within some of the regions where probable uORFs were predicted by the bioinformatics algorithms. The uORFs in the genes *ABCA1*, *ABCA3*, *ABCA7*, and *ABCA8* displayed a high level of ribosome coverage. Notably, a significant ribosome coverage peak was also detected at the site of the first uATG of *ABCA6* (−144), which was not connected to any uORF. An example of the Ribo-seq data analysis in the GWIPS-viz browser is disclosed in Figure 5 (on a case of the *ABCA1* gene); all results regarding uORFs are summarized in Table 3.

**Figure 5.** Ribosome profiling analysis in the GWIPS-viz browser, an example of the *ABCA1* gene. High concentrations of ribosomes can be seen at the sites of the predicted upstream ORF and beginning of the main ORF. Blue histograms represent Initiating Ribosome (P-site) Profiles from all studies, dark red—Elongating Ribosome (A-site) Profiles from all studies, red—Ribo-seq coverage data from all studies (Elongating Ribosomes—Footprints), and green—mRNA-seq coverage data from all studies (mRNA-seq Reads). mORF, main open reading frame; sATG, start ATG of the main ORF; uATG, upstream ATG; uORF, upstream open reading frame.


**Table 3.** Summary of the predicted uORFs in ABCA genes with Ribo-seq coverage data.

Abbreviations: Cov., coverage; No., number; uORF, upstream ORF; Ribo-seq coverage: 1 = Low, 2 = Medium, 3 = High; All positions are described in relation to the start ATG of the main ORF.
