*4.6. RG4 Analyses*

The DNA sequences downloaded were screened for the presence of RNA G-quadruplex-forming sequences by the three on-line prediction servers working with different prediction algorithms-G4CatchAll (Doluca lab; http://homes.ieu.edu.tr/odoluca/G4Catchall/) [56], G4RNA Screener (Scott Group Bioinformatics; http://scottgroup.med.usherbrooke.ca/G4RNA\_screener/) and QRGS Mapper (Ramapo College; http://bioinformatics.ramapo.edu/QGRS/index.php). The default settings of the programs were not changed and we followed the recommended cut-off levels. We adhered to the following result interpretation: the intersection of the results calculated by the G4CatchAll and G4RNA Screener were recorded as the most probable RG4-forming sequences. In the cases where no intersection of the two programs existed, the intersections of the G4CatchAll and QRGS Mapper or G4RNA Screener and QRGS Mapper were considered instead, and eventually recorded.
