*4.6. Multiple Sequence Alignment*

Sequences for NBD domains of ABC transporters were obtained from Uniprot [41] (human and mouse CFTR) or the PDB [42] (1B0U, 1L2T, 1G29, 1G6H, and 1JJ7). Alignments were performed with Jalview [43] using the T-Coffee [44] algorithm with default parameters.
