*4.1. Materials*

*E. coli* polar lipids (Cat. #: 100600C) and bovine liver polar lipids (Cat. #: 181108C) were from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Cholesterol, cholesteryl hemisuccinate (CHS), and *n*-dodecyl β-d-maltopyranoside (DDM) were from Anatrace (Maumee, OH, USA). The nickel–nitrilotriacetic acid (Ni–NTA) agarose resin was from Qiagen Toronto (Toronto, ON, Canada), while the calmodulin (CBP) affinity resin, zeocin, and ampicillin were from Agilent (Santa Clara, CA, USA). Imidazole, ε-aminocaproic acid, sucrose, yeast extract, tryptone, peptone, yeast nitrogen base (YNB), and ammonium sulfate were obtained from Wisent Bioproducts (St-Bruno, QC, Canada). ATP disodium trihydrate, Tris-(2-carboxyethyl)-phosphine (TCEP), sodium chloride, glycerol, ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(β-aminoethyl ether)-*N*,*N*,*N*′ ,*N*′ -tetraacetic acid (EGTA), sodium dodecyl sulfate (SDS), Ponceau S solution, sodium azide, Bradford reagents, Tween-20, magnesium chloride, calcium chloride, and all protease inhibitors were obtained from Bioshop Canada (Burlington, ON, Canada). Biotin, sodium cholate hydrate, l-ascorbic acid, ammonium molybdate, bismuth citrate, sodium citrate, methanol, ammonium hydroxide, hydrochloric acid, and acetic acid were obtained from MilliporeSigma (Oakville, ON, Canada). Dithiothreitol (DTT), Tris base, and Tris acetate were obtained from ThermoFisher (Ottawa, ON, Canada). Clarity Western enhanced chemiluminescence (ECL) substrates, 30% acrylamide, agarose, and ammonium persulfate were obtained from Bio-Rad (Hercules, CA, USA). Restriction enzymes were obtained from New England Biolabs (NEB) (Ipswitch, MA, USA), Promega (Madison, WI, USA), and ThermoFisher

(Ottawa, ON, Canada). The following media were used: yeast extract peptone dextrose (YPD), yeast extract peptone dextrose sorbitol (YPDS), minimal glycerol yeast nitrogen base (MGY), and minimal protease inhibitor buffer for yeast cell lysis (mPIB), consisting of 0.33 M sucrose, 0.3 M Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM EGTA, 100 mM ε-aminocaproic acid, and ddH2O to a final volume of 1 L and stored at 4 ◦C.
