*2.3. ABCA1*

The human ABCA1-202 transcript is 10,408 bp long and has 50 exons. The 5′UTR region covers 313 bps and is divided into two parts by one intron (Intron 1–2), which is the longest among ABCA genes with 24,163 bp. The most conserved sub-region of the 5′UTR was localized to −119 to −59. One uATG was found at −89. This uATG is highly conserved among vertebrates (category 5 = placental mammals + Reptiles and birds + coelacanth/ray-finned fishes); however, it shows a weak flanking sequence context and middle TIS score. One uORF starting at this uATG and overlapping with the main ORF was predicted. The most probable RG4-forming sequence was placed to −251 to −218. Six stem loops were predicted in this region. The ABCA1 protein was found to be expressed in all human tissues (45 tissues). The mode expression score was medium, and the expression was high in lung, stomach, and placenta.
