*4.7. RNA Secondary Structure Prediction*

RNAfold WebServer (University of Vienna; http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/ RNAfold.cgi) was employed to generate the optimal secondary structures for minimum free energy prediction in the form of dot-bracket notation, graphical visualizations colored by base-pairing probabilities and mountain plot representations of the minimum free energy (MFE) structures, the thermodynamic ensembles of RNA structures, and the centroid structures (Figure S5). Equivalent predictions made by RNAstructure Web Servers (Mathews group; http://rna.urmc.rochester.edu/ RNAstructureWeb/) were checked for comparison. The number of predicted stem loops within the whole 5′UTRs of the human ABCA genes was compared, counted and recorded.
