*4.4. Cell Migration Assays*

Cell migration was assessed using the Cell IQ (CM Technologies—intelligent cell analysis, Tampere, Finland) scratch assay. Cells were seeded in 24-well plates to reach 100% confluency the day of the assay. A scratch across the monolayer of the cells was carefully made using a 10-µL pipette tip, and the media was replaced with fresh prewarmed culture medium. Cells were treated with the inhibitors, as described above. Three image positions were selected from each well, and images were taken at 1-h intervals. Images were analyzed using the Cell-IQ analysis software (CM Technologies).
