*4.1. Cell Culture*

The MDA-MB-231 and MCF-7 human breast cancer cells were originally obtained from ATCC. They were cultured in Dulbecco's modified Eagle's medium (DMEM) (Lonza, Slough, UK) containing 4.5-g/L glucose and L-glutamine supplemented with 10% *v*/*v* fetal bovine serum (FBS) (Gibco®, ThermoFisher Scientific, Loughborough, UK) and 100-U/mL penicillin and 0.1-mg/mL streptomycin (sterile-filtered penicillin-streptomycin solution 100×, Biowest, Nuaillé, France). Cells were cultured in an environment of 95% air and 5% CO<sup>2</sup> at 37 ◦C.
