*4.2. Alignment and Tree Construction*

The final 174 protein sequences were aligned with MAFFT using the automatically assigned strategy, and other parameters set automatically by the MAFFT server, except raising the offset value to 0.123, which is the default value for the command line tool. This alignment was used to construct a tree using the Simply Phylogeny tool from ClustalW2, which was then visualised with the interactive Tree of Life [60]. The large number of

sequence names reduced the clarity of the figure, so were removed, but the branches were otherwise left intact.
