*4.8. Reagents*

All reagents used here were of the highest purity grade available. Forskolin and genistein were from Sigma-Aldrich (St. Louis, MI, USA); VX-809 and VX-770 were acquired from Selleck Chemicals (Houston, TX, USA). CFTR was detected with the mouse anti-CFTR monoclonal 596Ab, which recognizes a region of NBD2 (1204–1211) from CFFT—Cystic Fibrosis Foundation Therapeutics CFF Therapeutics [46] and calnexin with rabbit polyclonal anti-calnexin Ab SPA-860, from Stressgen Biotechnologies Corporation (Victoria, BC, Canada). Other specific reagents included: X-ray films (Kodak, supplied by Sigma-Aldrich); Complete Protease Inhibitor Mixture from Roche Applied Science (Penzberg, Germany); EZ-Link™ Sulfo-NHS-SS-Biotin from Pierce Chemical Company (Rockford, IL, USA).
