2.3.1. Water Absorption Capacity

The method described by Raghavendra et al. (2004) [36], with slight modifications, was used to determine the water absorption capacity (WAC) for the particle size of BF. From each sample, 1 g was measured and then was mixed into a 50 mL test tube with 10 mL distilled water. This mixture was left 30 min at room temperature and then placed in a centrifuge for 10 min at 2000 rpm (MPW-223e, MPW Med. Instruments, Warszaw, Poland). During this time, the following scientific phenomenon occurs: the water molecules hydrate the damaged starch, the glutenin and gliadin, and other components until molecules of starch and protein create hydrophilic interactions and hydrogen bonds with the molecules of water. When the hydration was over the supernatant was removed and the tubes were weighed.
