2.2.4. Determination of PLM Capacitance

The method used to determine PLM capacitance was as reported in a previous study [47]. Briefly, membrane capacitance was calculated using a calibration curve obtained by simulating the membrane capacitance with a discrete set of capacitances of known values, *Cn*, and measuring the corresponding output voltage, *Vlh*. The data obtained were fitted by the formula:

$$V\_{lh} = \frac{A \times \mathcal{C}\_n}{(B + \mathcal{C}\_n)}.$$

in which *A* and *B* are free parameters to be estimated by the fitting procedures. The values of parameters *A* and *B* were used to transform the *Vlh* value into capacitance data.
