**3. Results**

The experimental values of the surface charge densities of erythrocyte and thrombocyte membranes (control and fatal ethyl alcohol poisoning groups) versus pH are presented in Figures 1 and 2, respectively. We determined the surface charge density values based on experimental electrophoretic mobility data using Equation (1). All measurements were carried out at several pH values, using the basic electrolyte (0.155 M NaCl).

Figure 1 shows the surface charge densities of the control and fatal ethyl alcohol poisoning erythrocyte groups versus pH values. We observed a decrease in the positive charge and an increase in the erythrocytes membrane's negative charge after death by ethyl alcohol poisoning compared to control erythrocytes. A slight shift of the isoelectric point of membranes to lower pH values is visible.

Figure 2 shows the effect of pH on the surface charge density for the control and fatal ethyl alcohol poisoning thrombocyte groups.

**Figure 1.** The experimental surface charge density of erythrocytes vs. pH of the electrolyte solution •—control, —fatal ethyl alcohol poisoning.

**Figure 2.** The experimental surface charge density of thrombocytes vs. pH of the electrolyte solution: •—control, —fatal ethyl alcohol poisoning.

Fatal ethyl alcohol poisoning caused a decrease in the positive membrane charge and increased the thrombocyte group's negative charge compared with the control. Also, we observed a shift of the isoelectric point of the membrane to lower pH values.

Tables 1 and 2 show the isoelectric point values and the surface charge densities of the erythrocyte and thrombocyte membranes, as determined by microelectrophoresis. We

express the summarized results as the mean value with the designated standard deviation. We performed the analysis using standard statistical analysis.

**Table 1.** The isoelectric point and surface charge density values for red blood cells (RBCs) (control and fatal ethyl alcohol poisoning).


**Table 2.** The surface charge density and isoelectric point values for thrombocytes (control and fatal ethyl alcohol poisoning).

