2.2.2. Isolation of Thrombocytes from Plasma

The plasma was centrifuged at 4000 rpm for 8 min. Thrombocytes remained at the bottom of the tube after centrifugation. The plasma was removed from the vial. The platelets were washed three times with 0.155 M sodium chloride, as were the erythrocytes. Finally, the thrombocytes were added to the electrolyte solution (0.155 M NaCl). This solution was then used during measurements performed by the ELS technique.
