2.2.3. Determination of Conductance of Channel-Like Events

To determine conductance, we measured the amplitude of channel-like events by hand. The single channel data, filtered at 300 Hz, were obtained from at least three experiments (more than 100 single channel-like events) performed on different days. A histogram of the conductance amplitude distribution for each experiment was constructed and fitted by a Gaussian distribution function. Results are expressed as central conductance ±standard error (Λ<sup>c</sup> ± SE) and were evaluated by analysis of variance (ANOVA-Tukey test) and Student t test. A value of *p* < 0.05 was considered significant. The Gaussian distribution function, ANOVA test, Student t test, and the fitting procedures were performed using the GraphPad Prism 3 software (GraphPad PrismTM version 3.0, GraphPad Software, San Diego, CA, USA).

To determine the frequency (number of channel-like events in 60 s), any detection of channel events was counted as successful. Results are expressed as frequency ± standard deviation (F ± SD).
