*2.1. Materials*

The Ethics Review Board of the Medical University of Bialystok approved the conducted research (No. R-I-002/533/2010). During autopsies made at the Department of Forensic Medicine, Medical University of Bialystok, blood (pH ~ 6.9) was obtained from individuals who died due to fatal alcohol poisoning. The conducted experiment was based on ten selective fatal alcohol poisonings (5 men and 5 women; mean age 45.8 years, range 19–65). Blood was taken for tests from the femoral vein and placed in sterile containers, then sent to the Faculty of Chemistry, University of Bialystok for further research. The

obtained results were subjected to a comparative analysis with a control group obtained from live individuals at the Blood Donation Center in Białystok.

RBC isolation: The RBCs were isolated from 2 mL of whole blood by centrifugation for 8 min at room temperature at 900× *g*. The thrombocyte-rich plasma supernatant was removed, and the obtained erythrocytes were washed three times with 0.9% NaCl, then centrifuged for 15 min at 3000× *g*. After the final centrifugation, erythrocytes were resuspended in 0.9% NaCl, followed by microelectrophoretic measurement.

Platelet isolation: The thrombocyte-rich plasma was centrifuged for 8 min at 4000× *g*. The plasma supernatant was removed and discarded. Thrombocytes were washed three times with physiological saline solution (0.9% NaCl) and then centrifuged for 15 min at 3000× *g*. Then, thrombocytes were resuspended in 0.9% NaCl, and microelectrophoretic measurements were made.

The test solutions were made and all purification procedures were performed using ultrapure water from a Milli-Q11 system (18.2 MΩ cm, Millipore, Burlington, MA, USA).
