2.2.1. Isolation of Erythrocytes from Blood

The blood samples (2 mL volume) were centrifuged at 900 rpm for 8 min at room temperature. The red blood cells fell to the bottom of the tube, while the thrombocyterich plasma was removed from the system and further isolation was performed. The erythrocytes were washed three times with a 0.155 M NaCl solution at 3000 rpm for 15 min to obtain a clean erythrocyte-rich system. Finally, the washed red blood cells were suspended in an electrolyte solution (0.155 M NaCl) to prepare them for measurement via electrophoretic light scattering.
