*Article* **Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars**

**Victoria Vitkova 1, \*, Vesela Yordanova 2 , Galya Staneva 2 , Ognyan Petkov 1 , Angelina Stoyanova-Ivanova 1 , Krassimira Antonova <sup>1</sup> and Georgi Popkirov 3**


**Abstract:** Simple carbohydrates are associated with the enhanced risk of cardiovascular disease and adverse changes in lipoproteins in the organism. Conversely, sugars are known to exert a stabilizing effect on biological membranes, and this effect is widely exploited in medicine and industry for cryopreservation of tissues and materials. In view of elucidating molecular mechanisms involved in the interaction of mono- and disaccharides with biomimetic lipid systems, we study the alteration of dielectric properties, the degree of hydration, and the rotational order parameter and dipole potential of lipid bilayers in the presence of sugars. Frequency-dependent deformation of cell-size unilamellar lipid vesicles in alternating electric fields and fast Fourier transform electrochemical impedance spectroscopy are applied to measure the specific capacitance of phosphatidylcholine lipid bilayers in sucrose, glucose and fructose aqueous solutions. Alteration of membrane specific capacitance is reported in sucrose solutions, while preservation of membrane dielectric properties is established in the presence of glucose and fructose. We address the effect of sugars on the hydration and the rotational order parameter for 1-palmitoyl-2-oleoyl-*sn*-glycero-3- phosphocholine (POPC) and 1-stearoyl-2-oleoyl-*sn*-glycero-3- phosphocholine (SOPC). An increased degree of lipid packing is reported in sucrose solutions. The obtained results provide evidence that some small carbohydrates are able to change membrane dielectric properties, structure, and order related to membrane homeostasis. The reported data are also relevant to future developments based on the response of lipid bilayers to external physical stimuli such as electric fields and temperature changes.

**Keywords:** lipid bilayers; sucrose; capacitance; relative permittivity; dipole potential; membrane structure
