**4. Conclusions**

In this work, a systematic study on the surface potentials of Langmuir monolayers formed by the most abundant mammalian membrane phospholipids was performed to determine the values of their apparent dipole moments and to propose an improved protocol for estimating individual contributions to a three-layer capacitor model. Our methodology correlates the results from DFT molecular modeling with experimentally determined values using multiple linear regression. It can be applied to the study of other phosphatidylcholine derivatives (with similar hydration) with different hydrocarbon chain lengths and saturations. To be able to determine the parameters (µ w ⊥ /εw), ε<sup>p</sup> and ε<sup>a</sup> for other kinds of phospholipids, a similar approach should be carried out for their homologous series.

**Author Contributions:** Conceptualization P.D.-L. and J.K.; investigation, A.C.-B. and J.K.; data curation and visualization, J.K., A.C.-B. and A.W.; manuscript writing P.D.-L., A.C.-B. and A.W. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Acknowledgments:** This research was supported in part by PL–Grid Infrastructure.

**Conflicts of Interest:** The authors declare no conflict of interest regarding the publication of this article.
