*Review* **Wheat Germ Agglutinin—From Toxicity to Biomedical Applications**

**Gabriele Balˇ ˙ ciunait ¯ e-Murzien ˙ e˙ 1,2,\* and Mindaugas Dzikaras <sup>2</sup>**


**Featured Application: Wheat germ agglutinin has the potential for enabling and improving targeted drug delivery systems, anticancer drugs, and antibacterial and antifungal therapeutics due to its cytotoxic mechanisms and specific carbohydrate binding.**

**Abstract:** Wheat germ agglutinin is a hevein class *N*-Acetylglucosamine–binding protein with specific toxicity and biomedical potential. It is extractable from wheat germ—a low-value byproduct of the wheat industry—using well–established extraction methods based on salt precipitation and affinity chromatography. Due to its *N*-Acetylglucosamine affinity, wheat germ agglutinin exhibits antifungal properties as well as cytotoxic properties. Its anticancer properties have been demonstrated for various cancer cells, and toxicity mechanisms are well described. Wheat germ agglutinin has been demonstrated as a viable solution for various biomedical and therapeutic applications, such as chemotherapy, targeted drug delivery, antibiotic-resistant bacteria monitoring and elimination. This is performed mostly in conjunction with nanoparticles, liposomes, and other carrier mechanisms via surface functionalization. Combined with abundant wheat byproduct sources, wheat germ agglutinin has the potential to improve the biomedical field considerably.

**Keywords:** wheat; germ; wheat byproducts; agglutinin; WGA; toxicity; glycosylation; *N*-Acetylglucosamine; GlcNAc; carbohydrate
