*4.4. Identification of Markers, Watershed*

After marker identification (Euclidean Distance Transform and local maxima detection), the Watershed algorithm is applied, as shown in Figure 8. To improve the performance of the Watershed algorithm, the bandwidth h was defined by studying the range of variation of the cell size by means of a micrometer, a high precision gauge with a typical sensitivity of a hundredth of a millimeter.

**Figure 8.** Output images of Euclidean Distance Transform (**a**) and Watershed detected cells (**b**).
