2.4.2. Philippines

In the size class < 2 mm (*n* = 168 gnathiids) for Trial 1, five (3%) gnathiids molted into adult females and nine (5%) into males. In the size class < 2 mm (*n* = 153) for Trial 2, no gnathiids molted into adults. In contrast, for the larger size class > 2 mm for Trial 1 (*n* = 201), 20 (10%) gnathiids molted into females and 85 (42%) into males. In the size class > 2 mm (*n* = 165) for Trial 2, 37 (22%) gnathiids molted into females and 68 (41%) into males. In this study, newly metamorphosed males were first observed after day 1 in Trial 2 and day 2 in Trial 1, and no additional males appeared after day 5 in Trial 2 and day 7 in Trial 1. The mean number of days juvenile gnathiids molted into males for all treatments was 3.39 ± 0.75 and 3.23 ± 0.16 for Trials 1, and 2, respectively.

To test whether survival of gnathiids differed among temperature treatments, we used the same statistical methods as for the GBR data, with some modifications to the model. Temperature treatment was a fixed effect, and size class, life stage (male, female, or juvenile) and container (which the gnathiids were kept in) were treated as random effects. The ambient temperature of 28 ◦C was used as the baseline for analysis for Trial 1 and 30 ◦C for Trial 2. Assumptions of proportionality were met for both analyses (both Global tests: *p* > 0.05, see Tables S5 and S6 for results and Figures S6 and S7 for spline plots).
