*4.11. Cell Signaling Analysis*

HMC-1 cells were a kind gift from Prof. Soohyun Kim, Konkuk University, South Korea. HMC-1 cells were cultured in Iscove's modified Dulbecco's medium (IMDM) containing 10% FBS. HeLa cells were cultured in Dulbecco s modified Eagle s media (DMEM) containing 10% FBS. Cells were centrifuged at 800× *g* for 5 min at 4◦C, and they were lysed with NETN lysis buffer (100 mM NaCl, 20 mM Tris-HCl pH = 8.0, 0.5 mM EDTA, 0.1% NP-40, 5 mM NEM, 10 mM NaF, 1 mM Na3VO4, a protein inhibitor cocktail) for 15 min on ice. Cell lysates were centrifuged at 15,000× *g* at 4◦C for 10 min. Protein extracts were separated by SDS-PAGE and transferred onto PVDF membranes. Membranes were immunoblotted with the indicated antibodies, and the signals were visualized with ImageQuant™ LAS 4000 mini (GE Healthcare, Chicago, IL, USA). Anti-p-IkB (#2859), anti-IkB (#4814), anti-p-S6K (#9251), and anti-S6K (#9252) antibodies were purchased from Cell signaling. Anti-p-ERK (sc-7383), anti-ERK (sc-27129), and anti-IL-1RAcP (sc-376872) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-ST2 (#D065-3) were purchased from MBL (Woburn, MA, USA).

#### **5. Patent**

A patent application has been filed in South Korea (application number: 10-2020-0103135).

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/1422-0067/21/18/ 6953/s1.

**Author Contributions:** Conceptualization, S.B.P. and S.L.; investigation, S.B.P., S.-J.K., S.W.C.; resources, C.Y.C.; writing—original draft preparation, S.B.P.; writing—review and editing, S.L.; visualization, S.B.P.; supervision, S.L.; project administration, S.L.; funding acquisition, S.L. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the Basic Science Research Program (NRF-2018R1A2B6004367), the Science Research Center Program (NRF-2017R1A5A1014560), and the Bio & Medical Technology Development Program (NRF-2020M3A9E4039217) through the National Research Foundation of Korea (NRF) grants and the Next-Generation BioGreen 21 program (PJ01367602) through the Rural Development Administration.

**Acknowledgments:** We thank Yong-Soo Bae, Yoe-Sik Bae, and Seok Hee Park at Sungkyunkwan University for discussion.

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
