*2.3. Statistical Analysis*

Experiments on the SSF were performed in duplicate (*n* = 2) and two analytical replicates were performed on each batch (a = 2). Values present the mean value of the measurement ± SD. Raw data were statistically analysed using the software package MINITAB®® (version 17.1.0, Minitab Inc., State College, PA, USA). Analysis of variance (ANOVA) was determined using general linear model with a 95% confidence level followed by Tukey's multiple comparison test. A linear factorial regression model of the data was also conducted with the four inoculation methods as factor levels and days of SSF (2–8 days) as continuous control with protein concentration as output.
