2.4.3. ABTS Scavenging Activity

The determination of the antioxidant activity of a biosurfactant using the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay is based on the generation of the cationic chromophore radical obtained from the oxidation of ABTS by potassium persulfate [20]. The measurements were performed in triplicate and the inhibition activity was calculated based on the percentage of ABTS removed. Trolox and BTH were used as standards. The percentage of inhibition (I%) was calculated as described above for the DPPH scavenging activity.
