*2.4. Yeast Cell, Lactic Acid Bacteria and Coliform Bacteria Numbers*

Suspended yeast cells and lactic acid bacteria were counted via the dilution plating method, whereas coliform bacteria were counted via the MPN (most probable number) method.

The CFU (colony-forming unit) of suspended yeast was counted by culturing at 35 ◦C in yeast media agar (pH 5.8) containing 100 mg/mL chloramphenicol (Oxoid, Basingstoke, UK). Lactic acid bacterial colonies were counted by assessing acid formation at 37 ◦C in the MRS agar (pH 6.8) containing 50 mg/mL bromocresol purple (Oxoid, Basingstoke, UK). Coliform bacteria grown at 37 ◦C in lauryl tryptose broth (Oxoid, Basingstoke, UK) (pH 6.8) and the gas-forming bacteria were confirmed on green bile medium (Oxoid, Basingstoke, UK) (pH 7.4).
