2.3.2. Solid-State Fermentation (SSF): Substrate Preparation

Fifty mililiter Erlenmeyer flasks containing 1 g of rice hulls were autoclaved (121 ◦C for 20 min). After cooling, the moisture content of rice hulls was brought up to 60% by the addition of 0.6 mL of a sterile water solution of KH2PO<sup>4</sup> 2 g/L, NaCl 1 g/L, MgSO4, and 7H2O 1 g/L.
