*2.2. Analyses*

The fungal spore concentration was determined using a Bürker counting chamber. The final moisture content, the amount of fungal biomass harvested after SmF, and fungalbread product used as inoculum in Sc. IV were determined gravimetrically. Preparation of samples and measurement of the protein content by Kjeldahl digestion using a 2020 Kjeltec Digestor and a 2400 Kjeltec Analyser unit (FOSS Analytical A/S, Hilleröd, Denmark) was performed following the same procedure as in Gmoser et al. [25]. Total starch analysis was accomplished by α-amlylase/amylogluco-sidase/hexokinase method following the procedure provided in the Total Starch HK Assay Kit (Megazyme International Ltd., Bray, Ireland).
