*2.1. Microorganism and Its Maintenance*

*Aspergillus oryzae* DSM 1862 belongs to the collection of microorganisms of the Life Sciences and Biotechnology Department of the University of Ferrara and was purchased from the DSMZ (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany) company. It was propagated in potato dextrose agar medium (DIFCO, Wuerzburg, Germany), containing dextrose, 20 g/L, potato extract, 4 g/L, agar, 15 g/L added with chloramphenicol (Merck, Berlin, Germany), 50 mg/L for 72 h at 30 ◦C and stored in Petri dishes at 4 ◦C [19]. Amylase (CAS: 9000-90-2) lyophilized powder, ≥100 units/mg protein, was purchased from Merck, Berlin, Germany.
