*2.5. Acidity Value of Oils*

The acidity value (AV) of the olive oils was determined with the titration method reported in the Execution Regulation (EU) 2019/1604 and in the EN ISO 660: 2020 regulation.

The oil (1 g) was weighed into a flask and 50 mL of ethyl ether/ethanol mixture (1:1 ratio) and two drops of phenolphthalein ethanolic solution were added. The mixture was titrated with a KOH solution until the indicator changed color. The acidity was expressed as the weight percentage of free oleic acid present in 1 g of analyzed sample. The analyses were carried out in triplicate, and the average of the values are reported in Table 1 [22,23].

**Table 1.** Saponification, acidity, and peroxide values of EVO and NEFO oils.

