*2.5. Total Protein Quantification*

The total protein content was obtained by analyzing the samples by UV/Vis spectrophotometry at 595 nm with a colorimetric method by using the Kit Protein assay Biorad (Bio-Rad Laboratories Inc., Hercules, CA, USA) [22] and the bovine serum albumin (BSA) as standard (Bio-Rad Laboratories Inc., Hercules, CA, USA). A Beckmann DU-800 spectrophotometer was used.
