*2.2. Drp1 SiRNA*

The siRNA sequences were designed and synthesized by RibobioCo. Ltd. (Guangzhou, China). The hepatocytes were transfected with siRNA targeting Drp1 (siB121119100350-1-5) and its negative control (siB06525141910-1-5) using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). After 4 h of transfection, the hepatocytes were changed with complete medium.

### *2.3. ROS Level*

The intracellular ROS level was determined using 2, 7-dichlorofluorescein diacetate (DCFH-DA; Beyotime Institute of Biotechnology, Shanghai, China) by flow cytometry. L02 hepatocytes were treated with indicated chemicals and washed twice by cold PBS and loaded with 10 μM DCFH-DA at 37 ◦C for 40 min. After the incubation, the hepatocytes were washed again and analyzed by flow cytometry.

#### *2.4. Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) Levels*

L-02 hepatocytes were treated with different chemicals, and the supernatants were collected. ALT and AST levels were determined using the commercial kits (Jiancheng, Nanjing, China) according to the instructions. The optical density at 510 nm was measured using the multifunctional microplate reader.

### *2.5. Caspase-3 Activity*

Caspase-3 activity was determined using the commercial colorimetric assay kit. The hepatocytes were treated with indicated chemicals and washed twice with PBS, followed by lysis with the Caspase-3 Assay kit (Beyotime Institute of Biotechnology, China). The centrifugation was performed at 16,000× *g* at 4 ◦C for 10 min. The samples were then incubated with Ac-DEVD-pNA (substrate) at 37 ◦C for 2 h. The absorbance was measured and recorded by a microplate reader at 405 nm.
