3.2.2. Sample Amount

Until now, in most honey studies the employed amount of sample usually involves the use of several grams of honey [16,20,26]. Since one of the objectives of the work is to obtain a miniaturized methodology with a low sample, reagents and solvents consumption, two different sample sizes were evaluated: 0.1 g and 0.5 g diluted 1:10 w/v. Results are depicted in Figure 4b, for two honey samples varieties, HD and MF. As can be seen, in all cases responses were similar employing 0.1 g and 0.5 g, concluding that the use of only 0.1 g of honey were representative and homogeneous. Therefore, 0.1 g of honey sample and 1 mL of AW/MeOH were selected, allowing a miniaturization of the extraction VE-UAE procedure.

**Figure 4.** Optimization of the (**a**) extraction solvent; (**b**) sample size for two honey varieties (HD10 and MF32). \* Response multiplied ×5; \*\* multiplied ×20.
