2.3.2. Qualitative

The obtained sediment for quantitative analysis was centrifuged again and the supernatant was discarded. After vortexing, two drops (100 μL) of sediment were placed separately on a slide and distributed over an area of about 24 × 24 mm. Examination of pollen slides was performed using an optical microscope (400× or 1000×, as appropriate). The percentage of representation for each type of pollen was calculated by counting at least 500 pollen grains per sample. The pollen grains were classified as pollen type, as genus or a single species, when it was possible. Following the recommendations suggested by [13], the pollen frequency classes were determined.

### *2.4. Physico-Chemical Analysis and Color Determination*

Quality parameters including honey freshness (HMF and diastase content), moisture, electrical conductivity, pH and free acidity were determined in duplicate and following methodologies proposed by the International Honey Commission [14].
