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For each group, the asymmetries in the rCBF of the left and right hemispheres are illustrated in Figure 6 B. In the cortex, rCBF asymmetries were found in females. The asymmetry between left–right hemispheres was also observed in the hippocampus of NTg males and 3xTg females and the striatum of NTg females (paired *t*-test, *p* < 0.05).

## *3.6. Angiogenesis*

The middle cerebral artery (MCA) (Figure 7A,B) and the aorta (Figure 7C,D) was longitudinally measured at day 4, 5, 6, 7 after seeding in growth medium containing Matrigel. Sex did not modify MCA angiogenic growth either in NTg or 3xTg-AD mice, though female 3xTg-AD mice showed an enhanced (*p* < 0.01) growth from day 4 to 6 compared to female NTg. In the aorta, although NTg females showed an initially lower angiogenic growth than males, growth was significantly increased (*p* < 0.05) in 3xTg-AD females compared to 3xTg-AD males (Figure 7D) and NTg females (Figure 7C vs. 7D).

**Figure 7.** Influence of sex and genotype on the MCA and aortic angiogenic response. Analysis of neovessel growth progression in MCA from NTg (**A**) and 3xTg-AD (**B**) male and female mice. Representative images of neovessel sprouting (above) at day 7 (left) or 6 (right) and analysis of neovessel growth (below) in aorta from NTg (**C**) and 3xTg-AD (**D**) male and female mice. Red dotted lines represent the length of the longest vessel sprouting from the artery's outer surface (starting point). Results are the mean ± SEM from NTg, male *n* = 6–7, female *n* = 4–5; 3xTg-AD, male *n* = 10, female *n* = 5. Data were analyzed by two-way repeated measures ANOVA with Tukey post-test. \* *p* < 0.05, ## *p* < 0.01.
