*3.3. Ex Vivo Superfusion*

Figure 8 shows fractional dopamine release from the VTA. A p value was not calculated for the time factor (F (15.00, 104.0) = 16.41). There was no significant main effect neither for the treatment factor (F (1, 7) = 0.0008258, *p* = 0.9779), nor for the interaction between treatment and time (F (15, 104) = 0.5151, *p* = 0.9273). There was no significant difference between the groups at any other time point.

**Figure 8.** Fractional dopamine release from the ventral tegmental area. *n* = 4–5.

Likewise, Kp-8 did not influence fractional dopamine release from the NAc (Figure 9). However, there was a significant main effect for the time factor (F (3.134, 40.75) = 22.48, *p* < 0.0001). No significant main effect was found for the treatment factor (F (1, 13) = 0.0007717, *p* = 0.9783), and for the interaction between treatment and time (F (15, 195) = 0.4387, *p* = 0.9658). No significant difference could be detected at any specific time point between the groups.

**Figure 9.** Fractional dopamine release from the nucleus accumbens. *n* = 7–8.

As shown in Figure 10, Kp-8 increased fractional GABA release from the NAc. There was a significant main effect for the time (F (2.227, 17.82) = 60.49, *p* < 0.0001) and interaction (F (15, 120) = 7.395, *p* < 0.0001) factors. In the seventh fraction, following electrical stimulation, fractional GABA release was significantly higher from the Kp-8 treated brain slices than from the control tissue (*p* = 0.0039).

**Figure 10.** Fractional GABA release from the nucleus accumbens. \*\* *p* < 0.01 vs. control, *n* = 5.
