*2.2. Coating Preparation*

The elaboration of the polymeric coatings was conducted at room temperature. For the establishment of the unloaded polymer, chitosan was dissolved in 1% acetic acid with continuous magnetic stirring for 24 h at a concentration of 1.0% w/v. Then, PLGA was dissolved in DCM (25% w/w vs. chitosan) and subsequently loaded into a 1 mL syringe. This PLGA solution was added to the chitosan solution drop by drop using a 23-gauge needle, and the obtained chitosan–PLGA dispersion was stirred for 12 h for evaporation of the organic solvent DCM. In the case of the drug-loaded polymers, CHX (2% w/w vs. chitosan) or RIF (5% w/w vs. chitosan) were dissolved in DCM and the mixture with the solution of PLGA was added drop by drop slowly, to the chitosan aqueous solution under stirring for 12 h. In this time period, the organic solvent DCM evaporated with the subsequent formation of solid nanoparticles containing the biodegradable polymer and the corresponding antiseptic or antibiotic drug as a nanodispersion in the original aqueous solution of chitosan, which was applied for the homogeneous coating of the commercial mesh. According to the amount ratio of PLGA and drug, CHX nanoparticles contained 10% (w/w) of the drug, whereas those prepared with RIF contained 15% (w/w) of the bioactive drug.
