*2.4. Visualization of the PLGA-Nanoparticles Loaded Biocomposites*

The size and distribution of the nanoparticles from the original dispersion of nanoparticles in the solution of chitosan was analyzed under scanning electron microscopy (SEM), via the deposition of a few drops on the sample holder of the microscope. Moreover, samples from the different study groups (*n* = 3 each) were used to evaluate the homogeneity and distribution of the polymeric coating. The meshes were dehydrated in an increasing graded ethanol series (30%, 50%, 70%, 90%, and 100%, incubation for 15 min each), desiccated in a Polaron CPD7501 (Fisons Instruments, Ipswich, UK), gold-palladium coated, and visualized in a Zeiss DSM950 SEM (Carl Zeiss, Oberkochen, Germany).
