*2.1.* α*-Lac-Added Reconstituted IMF Preparation*

Bovine α-Lac powder (native form) was generously donated by Davisco Foods International, Le Sueur, MN, USA. The protein content and α-Lac level in the powder were more than 95% and 90%, respectively. Spray-dried IMF powder (stage 1, 1.4 g protein/100 mL, whey protein to casein ratio 60:40) was reconstituted into cooled, boiled milli-Q water, as per the instructions of the manufacturer. Finally, 100 mg of α-Lac was added into 100 mL of reconstituted IMF, and a magnetic stirrer was used for gentle mixing.
