2.5.1. Catalase Activity

The activity of the antioxidative enzyme, catalase, was evaluated using the Catalase Assay Kit from Cayman Chemical (Ann Arbor, MI, USA, catalog number 707002). Methanol was reacted with catalase in the presence of for the optimal concentration of H2O2. The production of the resulting formaldehyde was measured colorimetrically with Purpald (4-amino-3-hydrazino- 5-mercapto-1,2,4-triazole) at 540 nm.
