*2.1. Experimental Animals and Groups*

Male Wistar rats between 300 and 350 g were used. The animals were kept in acrylic cages under bioterium conditions at room temperature for a period of 12 h of light/12 h of darkness, with free access to food and water. Animals were randomly assigned to eight groups (See Table 1).



The diets are applied for eight weeks, while the protocol of moderate-intensity exercise is applied for five weeks. Diazoxide was administered for 14 days intraperitoneally at a dose of 35 mg/kg. Finally, the respective combinations were combined.

Diets were administered for eight weeks; the standard rodent chow® diet showed a caloric content of 336 cal/100 g with a proportion of 28.507% protein, 13.496% fat, and 57.996% carbohydrates. The high-fat diet contained 50% standard rodent chow® and 50% fat [2], showed a caloric content of 649.25 cal/100 g with a proportion of 14.05% protein, 69.5% fat, and 21.4% of carbohydrates (Mexican equivalent food system). The exercise protocol was applied for five weeks at moderate intensity (see Table 2) and diazoxide was applied at a dose of 35 mg/kg intraperitoneally for 14 days in the obese group, diazoxide was applied at the end of the obesity induction period, while in those subjected to the exercise protocol, the drug was applied during weeks four and five.


**Table 2.** Exercise protocol. Exercise protocol per week for groups: E; exercise, ED; diazoxide exercise, OE; obese exercise, OED; obese diazoxide exercise. The speed is displayed in meters per minute and the time for which this speed was applied is shown in parentheses.

All procedures with animals were carried out by the Federal Regulations for the Use and Care of Animals (NOM-062-ZOO-1999) issued by the Ministry of Agriculture of Mexico.
