**5. Conclusions**

Consumption of a probiotic product based on the three strains of *B. longum*, *L. casei*, and *L. rhamnosus* for 6 weeks in male amateur cyclists undergoing high-intensity and duration physical exercise was associated with a reduction of lipid-related oxidative stress biomarkers, without an increase in antioxidative enzymes. These findings suggest an antioxidant effect of the probiotic product on underlying interacting oxidative stress mechanisms and their modulation in healthy subjects.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2076 -3921/10/2/323/s1, Table S1: Sequences\_distribution; Table S2: Microbiome\_profile; Table S3: DESeq2\_differential-populationGenus.

**Author Contributions:** Conceptualization, F.J.L.-R. and V.Á.-G.; methodology, F.J.L.-R., A.J.L.-R., and V.Á.-G.; software, S.P.-P. and A.M.G.-M.; validation, D.V.-M., A.M.G.-M., and S.P.-P.; formal analysis, A.M.G.-M., M.S.M., E.C. (Eric Climent), S.G., D.R., and E.C. (Empar Chenoll); investigation, M.S.M., M.S.A.-R., D.V.-M., A.M.G.-M., and S.P.-P.; data curation, F.J.L.-R.; writing—original draft preparation, M.S.M., M.S.A.-R., and F.C.; writing—review and editing, M.S.M., M.S.A.-R., and F.C.; visualization, F.C.; supervision, V.Á.-G.; project administration, S.P.-P.; funding acquisition, F.J.L.-R. All authors have read and agreed to the published version of the manuscript.

**Funding:** We received funds from the CDTI agency of the Spanish Ministry of Economy and Competitiveness and European Regional Development Fund (ERDF), under the call of the Strategic Program of the Consortia of National Business Research (CIEN), project SMARTFOODS.

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committee of UCAM (CE101703).

**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.

**Data Availability Statement:** No new data were created or analyzed in this study. Data sharing is not applicable to this article.

**Acknowledgments:** The authors thank Marta Pulido for editing the manuscript and for editorial assistance.

**Conflicts of Interest:** Eric Climent, Salvador Genovés, Daniel Ramon and Empar Chenoll are employees of ADM-Biopolis. All other authors declare that they have no conflict of interest.

#### **References**

