*3.1. Body Weight and Food Intake*

Percent changes in body weight from the week before behavioral testing started and at the end of the study are presented in Figure 1. Overall, male mice weighed more than female mice, and within the female groups, GFAP-ApoE4 mice weighed less than the GFAP-ApoE3 mice (not shown). These observations were supported by main effects of strain, sex, and an interaction between sex and strain (all *p* < 0.02). By week 7, most mice lost 3–7% of their body weight, and the only significant differences were between E3 and E4 Sed-Con females, and between Sed-Con and Sed-Aox in the GFAP-ApoE4 females (all *p* < 0.05). By week 12, the loss was more pronounced (up to 13%), and the only significant differences were Ex-Aox GFAP-ApoE4 females losing less weight than Sed-Con (*p* < 0.05), while in males, the Ex-Aox groups lost the most weight (*p* < 0.05 only for GFAP-ApoE3). Food intake was not significantly affected by sex, strain or treatment (all *p* > 0.2) (not shown).

**Figure 1.** Minimal effects of exercise and/or antioxidant regimen over time on body weights of middle-aged GFAP-APOE3 and GFAP-APOE4 male and female mice (mice expressing the human apolipoprotein (Apo) E3 or E4 under glial fibrillary acidic protein (GFAP) promoter control). Each value represents mean ± SEM, *n* = 5–16 for body weights, and *n* = 3–7 for food intake. \* *p* < 0.05 vs. sexand strain-matched Sedentary-Control (Sed-Con) groups; # *p* < 0.05 comparing sex-matched Sed-Con GFAP-ApoE3 and GFAP-ApoE4.

#### *3.2. Behavioral Measurements*
