2.2.1. Determination of Particle Size Distribution by Laser Diffraction

A Mastersizer (Mastersizer 3000, Malvern Instruments Ltd., Malvern, Worcestershire, UK) was used as the laser diffraction particle size analyzer. The emulsions were prepared according to the formulation listed in Table 1, and they were cooled to room temperature before measurements. The refractive index ratios of sunflower oil and distilled water were assumed to be 1.472 and 1.333, respectively. The stirring speed was adjusted to 2100 rpm, and emulsions (approximately 50–100 μL) were added to 500 mL of aqueous dispersant (distilled water) until a laser obscuration value of 10–14% was obtained [20–22].
