**2. Materials and Methods**

### *2.1. Emulsion Preparation*

Oil-in-water (O/W) emulsions were prepared in the present study. Commercial sunflower seed oil was purchased and used as the oil phase. Polysorbate 80 (Crillet 4™) was kindly provided by Croda (Croda International Plc, Snaith, UK) and used as an emulsifier at a constant ratio of 2% (*w*/*w*) in all emulsions. The experiment was designed with the aim of obtaining emulsions with different droplet sizes. For this purpose, the oil/water phase ratios of emulsions (5%, 10%, 20%, 30%, 40%, and 50%) and homogenization times (3, 6, and 9 min) were varied and are presented in Table 1.

In the emulsion preparation, the total weight of each emulsion was kept constant at 30 g. Firstly, Crillet 4™ was dissolved in distilled water in a 50 mL Falcon tube. Then, sunflower oil was added to the tube and mixed. The mixture was homogenized with Ultra-Turrax (T-18 Digital Package, IKA, Staufen, Germany) at 10,000 rpm. The preparations were carried out in two repetitions, and the measurements were performed in triplicate.


**Table 1.** Oil/water phase ratios and homogenization times of the prepared emulsions.

\* The samples were also analyzed after one day of storage.
