Nano Sight Wave

The laser-based light scattering analysis of CD particles was performed with Nano Sight NS300 (Malvern, Worcestershire, UK), fitted with an O-ring top-plate. Nanoparticle tracking analysis (NTA) software was used to capture images and process data, representing the concentration, size distribution, and intensity of particles in the sample. Sample measurement was done in static mode using a capture time of 60 s and five repeats. The

camera level was adjusted to 11 so that all particles were visible. The same camera level was used for all the samples. A suitable detection level was selected for data analysis to limit the detection of non-particles and was between levels 4 and 12. The result for each sample was based on the average of five measurements obtained from the NTA and represented by the average particle concentration, average particle size (i.e., mean size), and mode size (i.e., the size that displays the highest peak).
