3.1.3. Synthesis of PGAγCyDArg3

The synthesis was carried out as for PGAβCyDArg2 with PGA (10 mg), DMTMM (19 mg), ArgOCH<sup>3</sup> (8 mg), DMTMM (20 mg) and γCyDNH<sup>2</sup> (67 mg).

<sup>1</sup>H NMR (500 MHz, in D2O) δ (ppm): 5.20–4.77 (m, H-1 of CyD); 4.32 (m, CH Arg); 4.23 (s, CH Glu); 4.12 (m, H-3A of CyD); 3.93–3.49 (m, H-3, -6, -5, -2, -4 of CyD and OCH<sup>3</sup> of Arg); 3.12 (γ-CH<sup>2</sup> Arg); 2.57–1.45 (β- and δ- CH<sup>2</sup> PGA, CH<sup>2</sup> Arg); 1.38 (m, CH<sup>2</sup> butyl chain of PGA), 1.21 (m, CH<sup>2</sup> butyl chain of PGA); 0.77 (m, CH<sup>3</sup> butyl chain of PGA).

Dimension (DLS, Z average): 29 ± 3 nm; PDI (DLS): 0.4; Zeta potential: 2.3 ± 0.5 mV (pH = 7.4).
