*3.5. Solubility Experiments*

DOX hydrochloride (0.017 M, water solution) was added to the solutions containing different concentrations (25 mg/mL, 12 mg/ML, 6 mg/mL) of all the polymers in phosphate buffer (50 mM, pH 7.4). The suspensions were sonicated for 3 min, incubated at 25 ◦C in the dark and centrifugated after 2 h. DOX was determined in the supernatant with UV/Vis spectroscopy, at 482 nm.
