*4.8. Data Analysis*

GraphPad Prism® (version 4.03 for Windows, GraphPad Prism Software, San Diego, CA, USA) was used for graphic illustrations and statistical analysis. The statistical tests used to study the significance of the results are described in the captions of the corresponding figures.

**Supplementary Materials:** The following are available online. Figure S1: Cholesterol content in A549 cells untreated (control), incubated with methyl-β-cyclodextrin (MβCD) for 30 min, and incubated with methyl-β-cyclodextrin (MβCD) for 30 min and thereafter in medium for 2 h. Figure S2: Effect of the BUD: HPβCD complex (green) and HPβCD (blue) on oxidant generation induced by H2O<sup>2</sup> + LPS after treatment for 2 h in non-depleted and sphingomyelin-depleted A549 cells. Figure S3: Effect of the BUD:HPβCD complex versus HPβCD on H2O<sup>2</sup> (**A**) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH•) (**B**) after 25 min (**A**) and 1 h of incubation (**B**). Figure S4: Cholesterol content in A549 cells incubated with the BUD:HPβCD complex, HPβCD or BUD for 2 h. Figure S5: HPβCD relative quantity in A549 extracellular medium after 2 h of incubation with the BUD:HPβCD complex (green bars) and HPβCD (blue bars). HPβCD was quantified using proton nuclear magnetic resonance spectroscopy. Figure S6: Oxidant generation kinetic in A549 cells after treatment with H2O<sup>2</sup> + LPS (1 mM + 100 µg/mL) for 6 h and effect of *N*-acetyl-l-cysteine (NAC) and Vit C on H2O<sup>2</sup> + LPS-induced oxidant generation. Figure S7: Akt phosphorylation induced by H2O<sup>2</sup> + LPS, H2O<sup>2</sup> and LPS in A549 cells after 2 h of incubation. Figure S8: Percentage of IL-8 released after A 549 cells pretreatment for 30 min with trichostatin (TSA) and incubation for 2 h with TNF-α in presence of BUD + HPβCD complex, BUD or HPβCD [68–71].

**Author Contributions:** Conceptualization, D.C., J.C.B. and M.-P.M.-L.; Methodology, J.C.B.; P.D.T.; B.E. and M.-P.M.-L. Investigation, J.C.B.; P.D.T.; B.E.; Resources, P.D.T.; B.E. and M.-P.M.-L.; Writing—Original Draft Preparation, J.C.B.; Writing—Review and Editing, J.C.B. and M.-P.M.-L.; Supervision, M.-P.M.-L.; Project Administration, D.C. and M.-P.M.-L.; Funding Acquisition, D.C., B.E. and M.-P.M.-L. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Acknowledgments:** JCB thanks WB and OJ for the BUD:HPβCD complex used in this study and V. Mohymont who provided dedicated technical assistance. This work was supported by Walloon Region (AEROGAL) and FRIA.

**Conflicts of Interest:** The authors declare no conflict of interest.
