PGA*γ*CyDArg5

<sup>1</sup>H NMR (500 MHz, in D2O) δ (ppm): 5.22–4.70 (m, H-1 of CyD); 4.32 (m, CH Arg); 4.23 (s, CH Glu); 4.12 (m, H-3-A of CyD); 4.05–3.32 (m, H-3, -6, -5, -2, -4 of CyD and OCH<sup>3</sup> of Arg); 3.12 (γ-CH<sup>2</sup> Arg); 2.59-1.46 (β- and δ- CH<sup>2</sup> PGA, CH<sup>2</sup> Arg); 1.37 (m, CH<sup>2</sup> butyl chain of PGA), 1.21 (m, CH<sup>2</sup> butyl chain of PGA); 0.78 (m, CH<sup>3</sup> butyl chain of PGA).

<sup>13</sup>C NMR (125 MHz, in D2O) δ (ppm): 24.4 (β-CH<sup>2</sup> of Arg), 27.7 (α-CH<sup>2</sup> of Arg), 31.3 (β-CH<sup>2</sup> of PGA), 40.4 (δ CH<sup>2</sup> butyl chain of PGA), 52.4 (CH Arg), 52.8 (C-2 of CyD and OCH<sup>3</sup> of Arg), 52.9 (CH of Glu), 60.0 (C-6 of CyD), 71.5 (C-3 of CyD), 72.0 (C-5 of CyD), 80.4 (C-4 of CyD), 101. 7 (C-1 of CyD), 160 (C=N of Arg), 173–174 (CNH PGA-CyD, PGA-Arg), 174.72 (CO methyl ester of Arg).

Dimension (DLS, Z average): 59 ± 6 nm; PDI (DLS): 0.6; Zeta potential: 37 ± 3 mV (pH = 7.4).
