PGA*β*CyDArg4

<sup>1</sup>H NMR (500 MHz, in D2O) δ (ppm): 5.22–4.80 (m, H-1 of CyD); 4.32 (m, CH Arg); 4.23 (s, CH Glu); 4.12 (m, H-3A of CyD); 3.93–3.50 (m, H-3, -6, -5, -2, -4 of CyD and OCH<sup>3</sup>

of Arg); 3.12 (γ-CH<sup>2</sup> Arg); 2.60–1.49 (β- and δ- CH<sup>2</sup> PGA, CH<sup>2</sup> Arg); 1.39 (m, CH<sup>2</sup> butyl chain of PGA), 1.20 (m, CH<sup>2</sup> butyl chain of PGA); 0.78 (m, CH<sup>3</sup> butyl chain of PGA).

<sup>13</sup>C NMR (125 MHz, in D2O) δ (ppm): 24.4 (β-CH<sup>2</sup> of Arg), 27.6 (α-CH<sup>2</sup> of Arg), 31.3 (β-CH<sup>2</sup> of PGA), 40.5 (δ CH<sup>2</sup> butyl chain of PGA), 52.34 (CH Arg), 52.8 (C-2 of CyD and OCH<sup>3</sup> of Arg), 52.9 (CH of Glu), 60.0 (C-6 of CyD), 71.4 (C-3 of CyD), 72.5 (C-5 of CyD), 80.3 (C-4 of CyD), 101. 7 (C-1 of CyD), 160 (C=N of Arg), 173–174 (C-NH PGA-CyD, PGA-Arg), 174.7 (CO methyl ester of Arg).

Dimension (DLS, Z average): 79 ± 8 nm; PDI (DLS): 0.6; Zeta potential: 45 ± 5 mV (pH = 7.4).
