3.2.1. Dynamic Light Scattering and Zeta Potential Measurements

Dynamic light scattering (DLS) and zeta potential (ZP) measurements were performed at 25 ◦C with a Zetasizer Nano ZS (Malvern Instruments, Oxford, UK) operating at 633 nm (He–Ne laser). The mean hydrodynamic diameter (d) of the NPs was calculated from intensity measurement after averaging the five measurements. The samples (1 mg/mL) were diluted in phosphate buffer (pH = 7.4) prepared in ultrapure water filtered (0.2 µm).
