*4.7. Evaluation of Inflammatory Cytokine (IL-8) Expression by Sandwich ELISA*

A-THP-1 cells in 96-well plates were incubated with H2O<sup>2</sup> + LPS with/without BUD, HPβCD, or BUD:HPβCD complex. A 4-fold dilution of the supernatant in RPMI-1640 medium was stored overnight at −80 ◦C. IL-8 cytokine levels in diluted supernatant were quantified using the Human IL-8/CXCL8 DuoSet ELISA kit (R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
