*4.14. Statistical Analysis*

The results are expressed as the average value ± standard deviation (St.Dev) of three independent experiments. Median-effect analysis was performed by CalcuSyn software version 2.11 (Biosoft, Cambridge, UK) to calculate the values of the concentration required to cause a 1% inhibition of cell growth (IC1) and for a 50% inhibition of cell growth (IC50) for each nanosponge formulation. Statistical analyses were performed on Prism software version 6 (Graph-Pad, La Jolla, CA, USA) using a Student's *t*-test and one-way analysis of variance (ANOVA) to calculate the threshold of significance as appropriate. Statistical significance was set at *p* < 0.05.

**Author Contributions:** Conceptualization, R.C. (Roberto Canaparo), F.T., L.S., and R.C. (Roberta Cavalli); Data curation, M.A. and F.F.; Investigation, M.A., F.F., R.S., and F.C.; Supervision, R.C. (Roberto Canaparo), C.D.P., F.T., L.S., and R.C. (Roberta Cavalli); Writing—original draft, M.A. and F.F.; Writing—review & editing, R.C.(Roberta Cavalli), L.S., and R.C. (Roberto Canaparo). All authors have read and agreed to the published version of the manuscript.

**Funding:** The authors gratefully acknowledge funding from the University of Torino (Grant "Progetti di Ricerca di Ateneo 2011" and Grant "Ricerca Locale 2019") and from MIUR (PRIN 2010–2011 NANOMED, code 2010FPTBSH).

**Conflicts of Interest:** The authors declare no conflict of interest.
