*3.4. Preparation of the Solid Inclusion Complex and Physical Mixtures with Excipients*

The kneading method in a 1:1 molar ratio was employed to prepare the inclusion complex of RSP with RM-β-CD. For this purpose, 0.2851 g of RSP and 0.9149 g of CD were weighed, and the mixture was pulverized in an agate mortar and triturated with 1.2 g ethanol:HCl 0.1 M solution (1:1, m/m). Then, the thick slurry was kneaded for 45 min, and during the process, a few drops of solvent were added to maintain a suitable consistency. Thus, the product obtained was dried at ambient temperature and then in the oven at 40 ◦C for 24 h. The dried kneaded product was pulverized and passed through a 75-µm size sieve. In addition, a physical mixture of RSP with RM-β-CD in 1:1 molar ratio was obtained by mixing in the agate mortar and pestle for 10 min in a solvent-free manner.

The physical mixtures of RSP/RM-β-CD inclusion complex and each excipient were prepared by mixing in an agate mortar with pestle for approximately 5 min in the ratio of 1:1 (m/m).
