4.4.1. Lactate Dehydrogenase Assay

A549 cells in 96-well plates were incubated with increasing concentrations of BUD, HPβCD, or BUD:HPβCD complex, or with H2O<sup>2</sup> + LPS with/without increasing concentrations of BUD, HPβCD, or BUD:HPβCD complex. The activity of lactate dehydrogenase (LDH) released by non-viable cells in the supernatant was quantified using the Cytotoxicity Detection KitPLUS (LDH) from Roche (Mannheim, Germany) according to the manufacturer's instructions.
