*2.1. Cell Culture*

Lymphoblastoid cell lines were cultured according to the manufacturer's instructions in Roswell Park Memorial Institute (RPMI)-1640 medium supplemented with 2 mM glutamine and 15% fetal bovine serum (FBS). Frozen LCLs went through three freeze-thaw cycles with liquid nitrogen before use in enzymatic assays or respirometry. Lymphocytes were cryopreserved and did not require cell culture. Thawed lymphocytes were treated with 0, 0.1, 0.5, or 1 µM rotenone for 10 min and then frozen at −80 ◦C.

#### *2.2. Material and Reagents*

Table 1 presents all the reagents with the associated supplier and catalog number that were used to generate data for this review.


**Table 1.** Vendor and catalog number information for reagents used in the proof of concept assays completed for this review.
