*Article Ginkgo biloba* **Seeds—An Environmental Pollutant or a Functional Food**

**Teodora Tomova 1,\*, Iva Slavova 1, Desislav Tomov 2, Gergana Kirova 1 and Mariana D. Argirova 1**


**Abstract:** *Ginkgo biloba* has been cultivated in Bulgaria since the end of the 19th century. Ividual specimens can be seen in almost every park. Females of the tree are considered contaminants of the landscape because their ripe seeds have a strong odor and are not utilized. We undertook this study to clarify whether ginkgo seeds of local origin can be converted from an unwanted and unused environmental pollutant into a source of beneficial compounds. Various analytical and chromatographic methods were used to quantify the major constituents and ten biologically active compounds in methanol seed extract. The results showed that the seeds are low in proteins (5%) and fats (1%); the seeds were also rich in unsaturated fatty acids and tocopherols. About 44% of nut starch was resistant to in vitro enzymatic hydrolysis. The amount of terpene trilactones in an aqueous-methanol seed extract was significantly higher than the number of flavonoids. Ginkgotoxin and ginkgolic acid were also found. The extract demonstrated weak antimicrobial activity against thirteen microorganisms. This study revealed that seeds of locally grown Ginkgo trees can be used as a source of biologically active substances. The chemical composition show similarity to those of seeds from other geographical areas.

**Keywords:** *Ginkgo biloba*; trace elements; starch; flavonoids; terpene trilactones; ginkgotoxin; ginkgolik acid; antimicrobial
