**5. Conclusions**

Genomic mining by combining bioinformatics analysis and functional screening provides opportunities to find out novel biocatalysts, such as lipases. The present study allowed the in silico characterization of 14 putative *C. vulgaris* lipases with different cellular localization. Membrane associated lipases were also detected and described for the first time in this species. The 14 lipases display an acyl hydrolase motif (GXSXG) and belong to

the α/β hydrolase lipase 3 family and GX class. These putative lipases could be candidates for metabolic engineering in a future study to improve this microalga lipid productivity. In this study, we also report, for the first time, a putative lysosomal acid lipase produced by a green microalga. Further investigation on the generated 3D models, such as docking studies and MD simulations, will provide important information on the substrate catalytic process and the binding characteristics and could be of interest to understand molecular mechanisms of the loss-of-function mutants leading to WD and CESD in humans.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/1660-339 7/19/2/70/s1, Table S1 Templates used for 3D model generation.

**Author Contributions:** Conceptualization, H.B.H. and A.K.; methodology, H.B.H. and M.D. (Mouna Dammak); software, H.B.H.; validation, S.A., I.F. and P.M.; formal analysis, H.B.H., A.K., M.D. (Maroua Drira) and M.D. (Mouna Dammak); investigation, H.B.H., M.D. (Mouna Dammak), A.K. and M.D. (Maroua Drira); resources, S.A.; data curation, M.D. (Maroua Drira) and M.D. (Mouna Dammak); writing—original draft preparation, H.B.H.; writing—review and editing, H.B.H., S.A., M.D. (Mouna Dammak) and A.K.; visualization, S.A., P.M. and I.F.; supervision, S.A.; project administration, H.B.H. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Acknowledgments:** The authors are grateful to the Tunisian Ministry of Higher Education and Scientific Research for financial assistance.

**Conflicts of Interest:** The authors declare no conflict of interest.
