*Article* **Effect of Simulated Gastrointestinal Tract Conditions on Survivability of Probiotic Bacteria Present in Commercial Preparations**

**Lidia Stasiak-Róza ´ ˙ nska \*, Anna Berthold-Pluta, Antoni Stanisław Pluta, Krzysztof Dasiewicz and Monika Garbowska**

> Department of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska St. 166, 02-787 Warsaw, Poland; anna\_berthold@sggw.edu.pl (A.B.-P.); antoni\_pluta@sggw.edu.pl (A.S.P.); krzysztof\_dasiewicz@sggw.edu.pl (K.D.); monika\_garbowska@sggw.edu.pl (M.G.)

**\*** Correspondence: lidia\_stasiak\_rozanska@sggw.edu.pl; Tel.: +48-225-937-539

**Abstract:** Probiotics are recommended, among others, in the diet of children who are under antibiotic therapy, or that suffer from food allergies or travel diarrhea, etc. In the case of toddlers taking probiotic preparations, it is highly recommended to first remove the special capsule, which normally protects probiotic strains against hard conditions in the gastrointestinal tract. Otherwise, the toddler may choke. This removal can impair probiotic survival and reduce its efficacy in a toddler's organism. The aim of this study was to evaluate the survivability of five strains of lactic acid bacteria from the commercial probiotics available on the Polish market under simulated conditions of the gastrointestinal tract. Five probiotics (each including one of these strains: *Bifidobacterium* BB-12, *Lactobacillus (Lb.) rhamnosus* GG, *Lb. casei*, *Lb. acidophilus*, *Lb. plantarum*) were protective capsule deprived, added in a food matrix (chicken–vegetable soup) and subjected under simulated conditions of the gastric and gastrointestinal passage. Strain survivability and possibility to growth were evaluated. Obtained results showed that, among all analyzed commercial probiotic strains, the *Lb. plantarum* was the most resistant to the applied conditions of the culture medium. They showed a noticeable growth under both in vitro gastric conditions at pH 4.0 and 5.0, as well as in vitro intestinal conditions at all tested concentrations of bile salts.

**Keywords:** probiotics; resistance; survivability; gastrointestinal passage; gu<sup>t</sup>
