*2.5. Statistical Analysis*

Results obtained were subjected to statistical analysis using StatGraphicPlus 4.1. software Statgraphics Centurion software (Version 17.1.12, Gambit Centrum Oprogramowania i Szkole ´n Ltd., Kraków, Poland). Analysis of variance (ANOVA) was conducted. The Tukey test was applied to compare the significance of differences between mean values at a significance level of α = 0.05. Mean values from three replicates (*n* = 3) were also calculated, and the standard deviation was added to the mean as ± SD.
