**5. Conclusions**

In response to the growing field of human gu<sup>t</sup> microbiome study, we describe the development, assembly and use of a simple, low-cost at-home stool collection kit for rural or low-resource settings where modern toilets with seats are unavailable. This method for the collection of stool specimens was feasible, generally acceptable, and has strong potential for similar or adapted stool collection procedures among adults residing in other rural or low-resource settings.

**Author Contributions:** Conceptualization, J.A.J.F. and C.D.K.; methodology, J.A.J.F. and C.D.K.; software, J.A.J.F.; validation, J.A.J.F.; formal analysis, J.A.J.F.; investigation, J.A.J.F.; resources, C.D.K.; data curation, J.A.J.F.; writing—original draft preparation, J.A.J.F.; writing—review and editing, C.D.K.; visualization, J.A.J.F.; supervision, C.D.K.; project administration, J.A.J.F.; funding acquisition, C.D.K. All authors have read and agreed to the published version of the manuscript.

**Funding:** This trial was funded by a Canadian Institutes of Health Research (CIHR) Project Grant (ID400771).

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Clinical Research Ethics Board at the University of British Columbia in Vancouver, Canada (H18-02610), and the National Ethics Committee for Health Research in Phnom Penh, Cambodia (273-NECHR).

**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.

**Data Availability Statement:** All relevant data are within the manuscript.

**Conflicts of Interest:** The authors declare no conflict of interest.
