*3.3. Type of Fluorescence (Exogenous or Autofluorescence)*

NIR autofluorescence (NIRAF) alone was used in 11 studies, whereas an exogenous fluorophore was employed in 14 studies. Lerchenberger et al. [54] compared the usefulness of autofluorescence with an exogenous fluorophore, while Ladurner et al. assessed the utility of both autofluorescence and exogenous fluorophore, although not comparatively [59]. Dip et al. [62] compared the use of white light alone with the use of both autofluorescence and white light in the identification of PGs. ICG was used in all but one of the studies using exogenous fluorophores. Enny et al. used 500 mg of fluorescein dye to produce fluorescence in the PGs [47]. The ICG dose was 5 mg in five studies and 2.5 mg in two studies. Two studies reported the administration of repeat doses to a maximum of 5 mg/kg/day [60,61]. The fluorescence system varied from study to study, with the Fluobeam 800 system (Fluoptics, Grenoble, France) being the most common (five studies). The system was not specified in two studies [48,56] (Table 3). An image showing the autofluorescence of the PGs (Figure 2A) and the sequence of ICG angiography (ICGA) (Figure 2B,C) is provided in Figure 2.

**Figure 2.** A sequence of images showing ICGA of two PGs (arrows) from A to C. (**A**) Autofluorescence of the PG before the injection of ICG. (**B**,**C**) Diffusion of the ICG contrast agent confirming a well-vascularized PG (Fluobeam LX®—Fluoptics©, Grenoble, France).


**Table 3.** Fluorescence type, dosage, timing, and fluorescence system.


**Table 3.** *Cont.*

AF—Autofluorescence; ICG—Indocyanine green; NA—not applicable.
