2.4.3. Adsorption Thermodynamics

To determine the adsorption temperature of chrysin on the Bi-MIPs, the effect of five different temperatures (10, 20, 30, 40 and 50 ◦C) on the adsorption capacity of chrysin by Bi-MIPs was evaluated. The Bi-MIPs were weighed (0.02 ± 0.0002 g) with an analytical balance and placed into a round of 50 mL conical flasks. After adding 20 mL of chrysin methanol solution, the Bi-MIPs were used to adsorb chrysin (1 mg/mL) in an 80 rpm constant temperature oscillator. The concentration of chrysin in the supernatant was analyzed using UV-vis absorbance. The equilibrium adsorption capacity was calculated using Equation (1). At the same time, to evaluate the adsorption temperature of chrysin on the Bi-NIPs, Si-MIPs and Si-MIPs, under the same operating conditions of the Bi-MIPs, the concentration of chrysin in the supernatant was analyzed using UV-vis absorbance. The equilibrium adsorption capacity was calculated using Equation (1).
