**4. Conclusions**

The Bi-MIPs were prepared by precipitation polymerization using methacrylic acid and acrylamide as functional monomers and ethylene glycol dimethacrylate as a crosslinking agent. Their physicochemical properties and chemical structures were analyzed and characterized. The Bi-MIP has excellent adsorption performance for chrysin in methanol solution and has a larger specific surface area and thermodynamic properties. The adsorption of chrysin on the Bi-MIPs followed the PSO kinetic model and Langmuir isothermal model, and the adsorption process was dominated by homogeneous monolayer adsorption. Compared with the Si-MIPs, the Bi-MIPs showed excellent adsorption performance and specificity in the adsorption process of chrysin and its analogs. At the same time, the Bi-MIPs showed excellent adsorption performance in multiple cycles and were conducive to the extraction and purification of an organic solvent-soluble bioactive component from natural products. Under the synergistic action of AM, the force of MAA on the hydrogen bonds, electrostatic and π-π stacking of chrysin increased. This synthetic method is green, efficient, eco-friendly and cost-effective. Thus, this paper provides new ideas and methods for the synthesis of high adsorption performance, green and safe molecular-imprinted polymers and build the foundation for the large-scale extraction and separation of the Bi-MIPs in chrysin.

**Author Contributions:** Methodology, L.L. (Long Li) and L.L. (Lanfu Li); formal analysis, L.L. (Long Li), X.L., G.C. (Guoning Chen) and Q.H.; investigation, L.L. (Long Li), L.L. (Lanfu Li), Q.H. and S.W.; data creation, L.L. (Lanfu Li), W.W., G.C. (Gege Cheng), Y.W., G.C. (Guoning Chen) and X.L.; writing original draft preparation, L.L. (Long Li), W.W.; writing review and editing, L.L. (Long Li), X.L. and

Q.H.; supervision, X.L.; project administration, Q.H., G.C. (Guoning Chen) and X.L. All authors have read and agreed to the published version of the manuscript.

**Funding:** The research was supported by the National Natural Science Foundation of China (31860192), the Natural Science Foundation of Guangxi (2020GXNSFBA159009), the China Postdoctoral Science Foundation (2020M683209), the Opening Project of Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control (2019KF28), the Scientific Research Foundation of Guangxi Minzu University (2019KJQD10), the Natural Science Funds of Guangdong Province (2021A1515012425), and the International Collaboration Programs of Guangdong Province (2020A0505100010).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.

**Conflicts of Interest:** The authors declare no conflict of interest.
