*2.5. Test Procedure*

Five microliters of TRFM-DEX Ab immunoprobe were added to the microwell, and then 150 μL of standard solution or sample solution were added. After reaction for 4 min, the test strip was inserted into the microwell for 5 min for chromatographic reaction. The test strip was taken out of the microwell, and the sample pad was quickly peeled off. The qualitative result was observed under an ultraviolet lamp (Figure 1C). The quantitative results were obtained by inserting the test strip into the fluorescence quantitative analysis reader (Figure 1C). The fluorescence intensity of the T/C lines were converted into the corresponding peak. The stronger is the signal, the larger is the peak area.
