*2.8. Atomic Force Microscopy*

Atomic force microscopy (AFM) was used for obtaining the microstructure of the blank nanoliposomes and naringenin-loaded nanoliposomes with a concentration of 1.0 and 1.25 mg/mL naringenin before and after 31 days of storage at 4 ◦C. A droplet of nanoliposome suspension was placed on to a newly cleaved silicon substrate and airdried for 30 min, and then picture of the naringenin-loaded nanoliposomes were recorded through AFM (C300, Nanosurf, Liestal, Switzerland). AFM images were recorded in tapping mode with a scan time of 3 s and scan points of 512 to obtain sufficient data for statistical analysis.
