*Article* **Development of** *Nervilia fordii* **Extract-Loaded Electrospun PVA/PVP Nanocomposite for Antioxidant Packaging**

**Peng Wen 1, Teng-Gen Hu 2,3, Yan Wen 2, Ke-Er Li 1, Wei-Peng Qiu 1, Zhi-Lin He 1, Hong Wang 1,\* and Hong Wu 2,\***


**Abstract:** An ethyl acetate extract from of *Nervilia fordii* (NFE) with considerable suppression activity on lipid peroxidation (LPO) was first obtained with total phenolic and flavonoid contents and anti-LPO activity (IC50) of 86.67 ± 2.5 mg GAE/g sample, 334.56 ± 4.7 mg RE/g extract and 0.307 mg/mL, respectively. In order to improve its stability and expand its application in antioxidant packaging, the nano-encapsulation of NFE within poly(vinyl alcohol) (PVA) and polyvinyl(pyrrolidone) (PVP) biocomposite film was then successfully developed using electrospinning. SEM analysis revealed that the NFE-loaded fibers exhibited similar morphology to the neat PVA/PVP fibers with a bead-free and smooth morphology. The encapsulation efficiency of NFE was higher than 90% and the encapsulated NFE still retained its antioxidant capacity. Fourier transform infrared spectroscopy (FTIR) and X-ray powder diffraction (XRD) analysis confirmed the successful encapsulation of NFE into fibers and their compatibility, and the thermal stability of which was also improved due to the intermolecular interaction demonstrated by thermo gravimetric analysis (TGA). The ability to preserve the fish oil's oxidation and extend its shelf-life was also demonstrated, suggesting the obtained PVA/PVP/NFE fiber mat has the potential as a promising antioxidant food packaging material.

**Keywords:** electrspinning; *Nervilia fordii* extract; nano-encapsulation; antioxidant activity
