3.2.2. Specificity

The specific results are shown in Table S2. DEX Ab exhibited a strong CR to triamcinolone, with a CR rate of 54.5%, which may be due to the similar structure of the Ag recognition site. The CR rates to betamethasone, prednisolone, and hydrocortisone were 24.0%, 14.0%, and 1.5%, respectively. The specificity results of TRFM-ICG were consistent with that of icELISA, and the CR rates to DEX, triamcinolone, betamethasone, prednisolone, and hydrocortisone were 100%, 62.5%, 31.3%, 20.8%, and 4.2%, respectively. The specificity results show that the established TRFM-ICG method can be used for the multiple targets' detection of five glucocorticoids.
