**4. Conclusions**

In conclusion, we have proved that naringenin could be encapsulated into nanoliposomes by an easy pH-driven method according to the reduction in its water-solubility after the pH changed to acidity, which causes the naringenin can be moved from the aqueous solution into the lipid bilayers within nanoliposomes, while the naringin-loaded nanoliposomes contained some free naringin due to its higher water-solubility at lower pH values. Experiments showed that the naringenin-loaded nanoliposomes were predominantly nanometric, negatively charged and exhibited relatively high encapsulation efficiency. Moreover, the naringenin-loaded nanoliposomes still maintain good stability during storage at 4 ◦C for 31 days, while they were unstable at high store temperatures. In general, an easy method for manufacturing naringenin-loaded nanoliposomes has been constructed that may help to develop novel food-grade colloidal delivery systems and apply to introducing naringenin or other lipophilic polyphenols into foods, supplements, or drugs.

**Author Contributions:** Conceptualization, M.C. and R.L.; methodology, R.L. and W.Z.; software, Y.G. and Y.Z.; validation, M.C., W.Z. and R.L.; formal analysis, R.L. and L.L.; investigation, M.C. and R.L.; resources, Y.G. and L.L.; data curation, Y.C. and W.Z.; writing—M.C. and R.L.; writing—review and editing, R.L. and W.Z.; visualization, Y.G., Y.C. and Y.Z.; supervision, W.Z. and J.L.; project administration, R.L. and J.L.; funding acquisition, R.L. and W.Z. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the National Natural Science Foundation of China (grant number 32001689), the Natural Science Foundation of Guangdong Province of China (grant number 2021A1515010901), the Natural Science Foundation of Hainan Province of China (grant number 320QN325), the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (grant number 1630122017016 and 1630122021006), Innovative Research Team Construction Project for Modern Agricultural Industry Common Key Technologies of Guangdong Province (grant number 2020KJ116 and 2020KJ117), and the 'Guangdong Special Support Plan' Science and Technology Innovation Young Talents Project (2019TQ05N133).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** All authors are thankful to their representative universities/institutes for the support and services used in this study.

**Conflicts of Interest:** The authors declare no conflict of interest.
