2.4.3. Short-Term Stability Studies

The physico-chemical stability of Hyt@tgels was determined upon 14-day storage at different temperatures (4.0 ± 0.5 ◦C and 25.0 ± 0.5 ◦C). At predetermined times, aliquots were centrifuged (12,000× *g*, 4 h, 20 ◦C) to separate nanoparticles from the hydrogel. All samples were analyzed for particle size, PDI, and % drug entrapment efficiency, and the results were compared with the initial values. The Hyt stability during storage was confirmed by HPLC analysis as described in Section 2.4.4 paragraph.
