*2.10. Determination of Aqueous Solubility*

The aqueous solubility of mangiferin and its glucoside derivative were examined as follows: each compound was vortexed in double-deionized H2O for 1 h at 25◦ C. The mixture was centrifuged at 10,000× *g* for 30 min at 25 ◦C. The supernatant was filtrated with 0.2 μM of nylon membrane and analyzed with HPLC. Based on their peak areas, the concentrations of the tested compounds were determined by using calibration curves prepared with HPLC analyses of authentic samples.
