2.7.2. Encapsulation Efficiency

Encapsulation efficiency (*EE*) was determined by separating encapsulated hydrolysates from free ones using an Amicon filter (Amicon Ultra-15, with molecular weight cutoff of 30 kDa; Millipore Corp., Cork, Ireland), followed by centrifugation at 3000 rpm for 10 min. Free and total hydrolysates were determined by calculating the protein amount using the Bradford method as described previously.

*EE* was determined according to following equation:

$$EE = \frac{\text{Total protein content} - \text{Amount of free hydrolysisate}}{\text{Total protein content}} \times 100\tag{4}$$
