Reducing Power

The reducing power was assessed as described by Canabady-Rochelle et al. [32], with modifications. An aliquot of 70 μL of yogurt supernatant (diluted 1:5 in phosphate buffer pH 6.6, 200 mM) was homogenized with 35 μL of potassium ferricyanide solution (1% *w*/*v*), and incubated at 50 ◦C for 20 min. Subsequently, 135 μL of distilled water, 33 μL of trichloroacetic acid (10% *w*/*v*) and 27 μL of ferric chloride (0.1% *w*/*v*) were added and after 10 min, the absorbance was measured at 700 nm. The reducing power of the sample was shown as the absorbance at 700 nm after subtracting the absorbance value from the blank, where a higher absorbance value indicates greater reducing power. The determination was carried out in triplicate.
