*2.7. Yogurt Bioactivity*

For the analysis of antioxidant and antihypertensive activities, the supernatant of yogurt was used, which was prepared according to Zhang et al. [30]. For that, 10 g of yogurt samples were centrifuged at 4330× *g* for 5 min at 4 ◦C, and then the supernatants were recentrifuged under the same conditions.

#### 2.7.1. Antioxidant Activity

ABTS Radical Scavenging Activity

The 2,2 -azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity was determined using the methods detailed by Zheng et al. [31], with modifications.

The stock solution of the ABTS radical was generated by incubating 7 mM ABTS with 140 mM potassium persulfate in the dark for 16 h at room temperature. Before use, the stock solution was diluted with phosphate-buffered saline (PBS, pH 7.4) to an absorbance of 0.70 ± 0.02 at 734 nm. A 50 μL aliquot of the yogurt supernatant (diluted 1:5 in PBS) was homogenized with 150 μL of ABTS, and after reaction at 30 ◦C for 6 min in the dark, the absorbance was measured at 734 nm. The ABTS radical scavenging activity was calculated as follows: [(*Ac* − *As*)/*Ac*] × 100, where *Ac* is the absorbance of the control, reagents without sample, and *As* is absorbance with sample. The determination was carried out in triplicate.
