2.7.7.1. DPPH Radical Scavenging Activity

To measure DPPH radical scavenging activity, 1 mL of each concentration of hydrolysates was added to 1 mL of DPPH solution (0.1 mM), followed by incubation for 30 min in the dark. The absorbance was read at 517 nm and the DPPH radical scavenging activity was calculated by the following equation [19]:

$$\text{DPPH} \,\text{radial\,\,scavenging\,\,activity\,\,(\%)} = \frac{A\_{\text{control}} - A\_{\text{sample}}}{A\_{\text{control}}} \times 100\tag{6}$$

where *Acontrol* and *Asample* are the absorbance of the control and sample, respectively.
