2.2.2. Preparation of Lemon Oil-Based Nanoemulsions (LEO-NEs)

The LEO-NEs were formed from LEO, a mixture of two surfactants (Tween-80 and Span-80), and deionized water. A procedure for the oil–water mixtures was followed to obtain 20 mL; the pre-emulsion was centrifuged for 5 min at 10,000 rpm using a high-speed homogenizer (F6/10, Jingxin, Shanghai, China). The homogenate was processed further by an ultrasonicator (JY92-11D, Jingxin, Shanghai, China). During the ultrasonication process, samples were put in ice water for a low-temperature environment.
