*2.3. Hyt-Loaded Hydrogel (Hyt@tgel) Preparation*

Injectable hydrogels were prepared according to the cold method as reported by [33]. Briefly, HA (100 mg) and Pluronic F-127 concentrations of 12–25% (*w*/*v*, 5 mL) were mixed in double distilled water at a temperature below 4 ◦C to form hydrogels. The polymer solution was left for at least 24 h to ensure the complete dissolution. Meanwhile, lyophilized Hyt-NPs (1, 5, and 10 mg) were added to the polymer dispersion and stirred for 1 h at 4 ◦C.

#### *2.4. Hyt@tgel Characterization*
