*2.7. Assay of Transglycosylation Activity*

To determine the transglycosylation activity of the *Pg*MA, β-CD was used as a sugar donor, and the molecules, which belonged to triterpenoids, saponins, flavonoids, or polyphenol glycosides, were tested as sugar acceptors. The reaction mixture containing 5% (*w*/*v*) β-CD, 5.6 μg/mL *Pg*MA, and 1 mg/mL tested molecules, dissolved in DMSO

with 50 mM of PB (pH 7), was incubated at 65 ◦C for 24 h. The reaction mixture was then mixed with an equal volume of methanol and analyzed with high-performance liquid chromatography (HPLC).
