*2.4. Pigments Determination*

A spectrophotometric method was used to determine the quantitative content of pigments in plants. The determination of pigments was carried out in an ethanol extract using 1 cm thick cuvettes in triplicate biological and analytical repetition. Optical density was determined at wavelengths of 665 nm, 649 nm, and 470 nm. Calculation of the quantitative content of chlorophylls and carotenoids was carried out according to the formulas [38], followed by conversion to g of fresh weight [39].
