*Article* **Enhanced Lead Phytoextraction by Endophytes from Indigenous Plants**

**Ilaria Pietrini 1, Martina Grifoni 2, Elisabetta Franchi 1, Anna Cardaci 1, Francesca Pedron 2, Meri Barbafieri 2, Gianniantonio Petruzzelli 2 and Marco Vocciante 3,\***


**Abstract:** Lead (Pb) is one of the most common metal pollutants in soil, and phytoextraction is a sustainable and cost-effective way to remove it. The purpose of this work was to develop a phytoextraction strategy able to efficiently remove Pb from the soil of a decommissioned fuel depot located in Italy by the combined use of EDTA and endophytic bacteria isolated from indigenous plants. A total of 12 endophytic strains from three native species (*Lotus cornicolatus*, *Sonchus tenerrimus*, *Bromus sterilis*) were isolated and selected to prepare a microbial consortium used to inoculate microcosms of *Brassica juncea* and *Helianthus annuus*. As for *B. juncea*, experimental data showed that treatment with microbial inoculum alone was the most effective in improving Pb phytoextraction in shoots (up to 25 times more than the control). In *H. annuus*, on the other hand, the most effective treatment was the combined treatment (EDTA and inoculum) with up to three times more Pb uptake values. These results, also validated by the metagenomic analysis, confirm that plant-microbe interaction is a crucial key point in phytoremediation.

**Keywords:** soil remediation; phytoextraction; mobilizing agents; microbial endophytes; lead pollution
