2.3.5. Flavonoid Content

Flavonoid content (FC) in the samples was determined by a spectrophotometric method reported in literature with some modifications [20]. In a volumetric flask (5.0 mL), 0.5 mL of LOMW and 0.150 mL of NaNO2 (5.0 % *w*/*v*) solution were mixed. After 6 min, 0.300 mL of a 6.0 % (*w*/*v*) AlCl3 was added and after 5 min, 1.0 mL of NaOH (1.0 mol <sup>L</sup>−1) was also added to the mixture by immediately measuring the absorbance (510 nm) against a control solution. FC in LOMW was expressed as milligrams of CT per gram of sample, by using a calibration curve of CT (Standard solutions concentration of CT equal to 10.0, 25.0, 50.0, 75.0, 100.0 μM). Each measurement was performed in triplicate and data expressed as means (±SD).
