*2.9. Lipid Extraction*

Total lipids were extracted using a modified Bligh and Dyer method [26]: lyophilized samples (200 mg) were mixed with 12 mL of a solution of chloroform:methanol (2:1) and 3 mL KCl (0.88%), shaken, and centrifuged at 5140× *g* for 5 min. The lower phase was set aside, and the upper phase was subjected to further extraction with one volume of a solution of chloroform:methanol (2:1, *v*/*v*). After phase separation, the lower phase was isolated and added to the first one, and mixed with one-quarter volume of a solution of methanol:water (1:1, *v*/*v*). The lower phase was dried using nitrogen and analyzed for lipid composition.
