2.2.1. Anth Quantification

Leaf Anth was quantified with Dualex 4 (France), a new multifunctional blade measurement tool that calculates the absorbance of plants in the green region to obtain Anth (μg·cm<sup>−</sup>2). This tool can also accurately measure leaf Chl, surface flavonoid, and nitrogen content and is easy to use for real-time and non-destructive measurements. Each leaf was measured 10 times to obtain the representative value of Anth. The Anth measurement of healthy leaves was not required, except in the samples from the leaf vein; in infected leaves, Anth was only measured in parts that had turned red.
