*4.11. Cell Wall Degradability*

The CW degradability was assayed on 5 mg of crude CWs hydrolyzed with a cocktail of hydrolytic enzymes, containing 1% macerozyme (R10, from *Rhizopus* sp., Duchefa, EC number 232-885-6), 1% driselase (from *Basidiomicetes*, Sigma, EC number 286-055-3), 1% cellulase (Onozuka R10, from *Trichoderma viride*, Phytotechnology Laboratories, EC number 3.2.1.4), and 1% endo-polygalacturonase (M2, from *Aspergillus aculeatus*, Megazyme, EC number 3.2.1.15), in 20 mM sodium acetate (pH 4.8) (Fornalé et al., 2012). Samples were incubated at 37 ◦C by shaking. Aliquots of the CW hydrolysate were collected at different times, and 10 μL of 99% formic acid (Panreac) was added to stop the enzymatic digestion. The total sugars were quantified by the phenol-sulfuric method [105].
