*4.6. Electrophoretic Analysis*

Isoelectric focusing was performed on a Pharmacia Multiphor II system using Ampholine PAGplate (pH = 3.5–9.5) polyacrylamide gels according to the manufacturer's instructions (GE Healthcare). Peroxidase isoenzymes were stained with 4-methoxy-αnaphthol (4-MN) in the presence of H2O2.

SDS-PAGE was performed on 10 % (*w*/*v*) polyacrylamide gels using a MiniProtean® 3 Cell electrophoresis kit (Bio-Rad Laboratories, Barcelona, Spain) and a pH 8.8 electrophoresis buffer composed of 192 mM glycine and 25 mM Tris containing 0.1% SDS. SDS-PAGE was performed at 200 V for 40–45 min at room temperature. Proteins were stained with a Plus One Silver Staining kit, according to the manufacturer's instructions (GE Healthcare).
