*2.8. 18F-DPA-714 Quantification*

Quantification of 18F-DPA-714 binding was estimated through the Logan plot, where the distribution volume (VT) was approximated by the graphical analysis [39,40]. Logan plot analysis was based on the 30th to the 60th minutes of data [41]. For the bloodsampling cohort, the distribution volume VT was calculated from the metabolite-corrected AIF, denoted as VT,AIF. The distribution volume was also calculated from the metabolitecorrected IDIF, denoted as VT,IDIF and compared to VT,AIF. For each subject, nine target regions were chosen from the Freesurfer segmentation and calculated for VT,AIF and VT,IDIF. For the nonblood-sampling cohort, VT,IDIF was calculated with the same steps described. The distribution volume with respect to the reference region, denoted as VT,REF, was also computed with the cerebellum time-activity curves using the Logan graphical analysis.
