2.1.1. Inoculations

Additional inoculations of *Puccinia striiformis* were performed in April and May (25 April 2019; 12 May 2019). To establish high disease infections, the inoculations were repeatedly performed by applying a spore suspension to the plants immediately after rainfall incidences. Inoculations were timed to forecasted infection risks after the xarvio field manager (BASF Digital Farming GmbH, Münster, Germany). The spore suspension was applied with a garden pump sprayer and contained 8 × 10<sup>4</sup> spores/mL. Two liters of spore suspension were homogeneously applied over one plot.
