2.1.3. Solid Pigments

*Scytalidium cuboideum* pigment (dramada) crystals were precipitated by applying 200 mL of liquid nitrogen to a solution of 100 mL of concentrated acetone extract from *S. cuboideum* following the method stated by [15]. The acetone carrier differed from the traditional DCM as its melting point (−95 ◦ C) was preferred for the crystallization by precipitation method. After the crystals were formed in the cold solvent, they were filtered with the use of 415 Whatman filter paper (VWR). The crystals were then air-dried and placed in a glass vial. This method has been shown to result in crystals of high purity [15,55], with samples tested for purity in previous work [55].

To obtain solid xylindein from *Chlorociboria* spp., 15 mL of standardized *Chlorociboria* spp. extract in DCM was placed in a 30 mL ACE borosilicate glass vial and left uncovered to fully evaporate. Then, another 15 mL of extract was added. This process was repeated 15 times to form a solid pigment layer attached to the glass of the vial. Once the last fill of DCM extract was completely evaporated, 10 mL of acetone was added to the vial and it was closed with a non-evaporative polyseal-cone-lined cap. The vial was then shaken for one minute by hand before the mix was filtered using a VWR glass funnel equipped with a 415 Whatman filter paper to collect solid pigment. The acetone wash was repeated until the solid pigment was completely removed from the glass vial. After finishing this process, the filter paper was left to dry overnight. The resulting solid pigment was removed from the filter paper with the use of forceps and stored in a borosilicate glass vial. Solidified xylindein collected via this method does not yield a pure compound [56]; however, a standardized purification methodology has not yet been developed, though methods are in development [57].
