7.2.1. Nuclear Magnetic Resonance (NMR)

Nuclear Magnetic Resonance (NMR) is one of the most often used magnetic techniques for studying pigments of fungal origin. Whose basis is founded on the assumption that all nuclei with an odd number of protons and neutrons have a magnetic moment and inherent angular momentum or have a spin greater than zero [57]. As a result, this approach is primarily used to extract physical, chemical, electronic, and structural details on molecules.

By applying NMR on fungal pigments, it is possible to know different conformational aspects, such as the following [35,37]:


The use of this technique has helped over the years to explain some of the many structural details that pigments provide, especially those produced by fungi and some plants. NMR is called a low invasive procedure, which means that the effect it has on the molecular structure of pigments is comparatively low, allowing it to rule out the possibility that they are affected by energetic influences [58,59]. It is widely used in the study of the stability and reactivity of conformational isomers, which would be almost impossible to conduct without its inclusion. The proton (1H), 13C, and 15N are among the most common nuclei for its realization [35,38,60].
