*2.4. Biological Assays*

Compounds were tested for their antimicrobial activity against four fungi (*Candida albicans*, *Mucor hiemails*, *Pichia anomala*, *Rhodotorula glutinis* and *Schizosaccharomyces pombe*), four different Grampositive bacteria (*Bacillus subtilis*, *Micrococcus luteus*, *Mycobacterium smegmatis* and *Staphylococcus aureus*), and three Gram-negative bacteria (*Chromobacterium violaceum*, *Escherichia coli* and *Pseudomonas aeruginosa*), using oxytetracycline as a positive control against Gram-positive and Gram-negative bacteria, while nystatin was used as an antifungal positive control. Besides, cytotoxicities of the compounds against seven mammalian cell lines (human endocervical adenocarcinoma KB 3.1, breast cancer MCF-7, lung cancer A549, ovary cancer SK-OV-3, prostate cancer PC-3, squamous cancer A431, and mouse fibroblasts L929) were determined by the microculture tetrazolium test (MTT) method, using epothilon B as the positive control. Both bioactivity assays were performed following our standard protocols [15].
