*2.5. Phylogenetic Study*

The phylogenetic analysis was carried out based on the combination of the ITS, LSU, *rpb2*, and *tub2* sequences of the strain of *Jugulospora vestita* studied here and selected members belonging to the Sordariales, with *Camarops amorpha* SMH 1450 as outgroup. Each locus was aligned separately using MAFFT v. 7 [16], manually adjusted in MEGA v. 6.06 [17], and the individual gene phylogenies were checked for conflicts before the four gene datasets were concatenated [18,19]. The Maximum-Likelihood (ML) and Bayesian Inference (BI) methods were used in a phylogenetic analysis including the four loci concatenated as described by Hernández-Restrepo et al. [20]. Bootstrap support (bs) ≥ 70 and posterior probability values (pp) ≥ 0.95 were considered significant [21]. The alignment used in the phylogenetic analysis was deposited in TreeBASE (S26889).
