2.3.3. Embryo Photomotor Response (EPR) Behavior

Embryos were assessed at 24 hpf using a custom photomotor response analysis tool [62] in plate. The light cycle consisted of 30 s of dark background, a short light pulse, followed by a second light pulse nine seconds later and 10 more seconds of dark. Every exposure plate had 850 frames of digital video recorded from below at 17 frames per second, with white LED and infrared lights above. Recorded periods were truncated at the beginning and end of the experiment to ensure the same recorded period was compared.
