*2.6. Laccase-Like Activity Assay*

The laccase-like activity in the culture filtrate was determined as described by previous studies using 2,2 -azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) as the substrate [28–31]. Briefly, 200 μL of culture filtrate was added to the enzyme assay solutions (100 mM acetate buffer, pH 5.0), resulting in a final concentration of 1 mM of ABTS. ABTS oxidation was monitored based on the increase in A420 (ε<sup>420</sup> = 36,000 M−<sup>1</sup> cm−1). One unit of enzyme activity was defined as the amount of enzyme required to oxidize 1 μmol of ABTS per minute at 25 ◦C.
