*3.2. Genome Sequencing*

The genome of *A. resinae* KUC3009 was sequenced using a combination of PacBio and Illumina HiSeq reads with a 140x coverage. The genome assembly was approximately 30.11 Mb long and included 35 contigs with an average length of 183,235 bp (Table 1). The quality and completeness of the assembly were evaluated via BUSCO analysis. This analysis is routinely used to cross-analyze gene contents based on evolutionarily informed expectations of gene content [15]. Interestingly, 98.9% of the 1315 groups of genes required for the correct assembly of ascomycetes were present in *A. resinae* contigs, indicating that the *A. resinae* genome assembly was highly robust (BUSCO results are available in Supplementary File, Table S1). Only 0.4% and 0.7% of the gene groups were fragmented or missing, respectively.

The genomic features of *A. resinae* KUC3009 were compared with those of its sequenced relatives in the *Myxotrichaeae* family. The genome size of *A. resinae* KUC3009 (30.11 Mb) is similar to that of *A. resinae* ATCC 22711 (28.63 Mb) but smaller than that of *Oidiodendron maius* Zn (46.43 Mb) (Supplementary File, Table S2) [34]. For reference, the genome size of *A. resinae* was below the average genome size of common ascomycetes [35,36]. All species shared very similar genomic G+C content (i.e., approximately 47%). The average shared identity of three strains at the nucleic acid level was obtained with the OrthoANI calculator (Supplementary File, Table S2) [16]. The sequenced *A. resinae* strains were genetically closer to each other but were relatively distant from *O. maius*.


**Table 1.** Genome assembly statistics for *A. resinae* KUC3009.
