*2.3. Genome Analysis, Annotation, and Phylogenetic Analyses*

The completeness of the draft genome assembly was evaluated with BUSCO v3.0.2 using 1315 core genes of the Ascomycota dataset [15]. Genomic similarity comparisons between *A. resinae* and other strains were performed using orthoANI [16]. After the draft genome was analyzed, the gene locations were predicted using Maker (v2.31.8) [17]. tRNA and rRNA genes were predicted using tRNAscan (v1.4) and barrnap (v0.7, https: //github.com/tseemann/barrnap, accessed on 1 November 2018), respectively [18]. The functions of the predicted genes were annotated using Protein BLAST+ (v2.6.0), after which the annotated genes were classified according to KOG analysis [19]. Gene clusters related

to secondary metabolism were analyzed using antiSMASH Fungi v6.0 and secondary metabolite regions were identified using a "relaxed" strictness [20]. Lastly, Signal P5.0 was used to predict the presence of the signal peptide in translated products from the putative genes [21].

The internal transcribed spacer (ITS) region was selected for phylogenetic analysis of the selected fungus. The ITS region was amplified using the ITS1F (5 -CTT GGT CAT TTA GAG GAA GTA A-3 ) and LR3 (5 -CCG TGT TTC AAG ACG GG-3 ) primers. Polymerase chain reaction (PCR) was performed on a Bio-Rad MyCycler (Hercules, CA, USA) with the following protocol: initial denaturation at 95 ◦C for 5 min; 34 cycles at 95 ◦C (30 s), 55 ◦C (30 s), and 72 ◦C (30 s); final 5-min extension at 72 ◦C. DNA sequencing was carried out by Macrogen (Seoul, Korea) using the Sanger method with a 3730xl DNA analyzer (Life Technologies, Carlsbad, CA, USA). The ITS sequences were deposited in the GenBank database under accession numbers JN033458.2. The obtained ITS sequences were proofread and edited using reference sequences obtained from the GenBank database using MEGA v7.0, after which multiple alignments were conducted using MAFFT v7.130 [22,23]. The sequence alignments were manually modified when necessary. Additionally, a phylogenetic tree was constructed based on the ITS sequences of *A*. *resinae* and *Cladosporium*-like species using the "randomized accelerated maximum-likelihood" (RAxML) model coupled with the GTR+G evolution model and 1000 bootstrap replicates [24,25].
