*3.3. Genome Annotation*

By utilizing several different gene predictors, we found that the *A. resinae* genome contained 9638 genes, 298 tRNAs, and 228 rRNAs (Table 1). The gene density was 3.20 genes per 10 kilobases (kb), and the predicted average protein size was 465 amino acids. The genes typically exhibited exons and introns with average lengths of 452 and 86 bp, respectively. Moreover, each gene contained an average of 3 exons. Additionally, the average length of the predicted proteins was 465 amino acids.

The predicted proteins from the genes were annotated and then functionally classified using eukaryotic orthologous group (KOG) analyses (Figure 2). The annotated proteins were classified into the following categories: "intracellular processes," "metabolism," "information storage and processing," and "poorly characterized function" [37]. The results indicate that the proportion of the genes involved in the intracellular processes category was the highest, whereas the other categories were relatively insignificant. Among the metabolism category, the number of genes involved in lipid metabolism and the transport was slightly higher than that of the genes involved in carbohydrate metabolism and transport. *Cladosporium resinae*, the former name of *A. resinae*, is a common jet oildeteriorating microorganism, thus highlighting its high capacity to degrade saturated hydrocarbons [38,39]. Consistent with previous studies, gene annotation identified homologs of alkane degradation enzymes, such as long-chain alcohol oxidase [40]. These results indicated that the genes and enzymes of *A. resinae* make this fungal species potentially well-suited for bioremediation.

**Figure 2.** Eukaryotic orthologous group (KOG) distribution of predicted proteins from the *A*. *resinae* KUC3009 genome.
