2.3.2. Exposure Protocol

Six concentrations of solidified xylindein from *C. aeruginosa* and dramada from *S. cuboideum* (as described in Section 2.1.3) were compared to aniline (≥99.5% ACS grade, Sigma-Aldrich). Tested concentrations ranged from 50 to 2.32 mM for dramada and aniline. Roughly the same concentrations were used for xylindein; however, as yet no method exists that results in a pure compound, so tested concentrations ranged from 28.42 mg/mL (~50 mM) to 1.27 mg/mL (~2.32 mM). Compounds were exposed in 96-well plates, with one embryo per well loaded at 6 hpf and 100 ul of exposure solution. There were a total of 32 embryos per concentration across multiple plates, with 8 embryos exposed per concentration per plate.
