2.2.3. Assessment Protocol

The assessment method was modified from Truong, Harper and Tanguay [60]. At 24 hpf, developmental stages and spontaneous kinetics were observed over a two-minute period. At 120 hpf, embryo morphology, including body axis, ocular perceiver, snout, jaw, notochord, heart, brain, somite, fin, yolk sac, trunk, circulation, pigment, swim bladder and behavioral endpoints (motility, tactile replication), was observed and recorded. Mortality rate was also recorded at 24 and 120 hpf. The assessments were conducted in a binary form, as present or not present.
