2.3.4. Larval Photomotor Response Behavior (LPR)

Embryos were assessed at 120 hpf using a Zebrabox behavior chamber (ViewPoint Life Sciences, Montreal, CA, USA) with an infrared backlight stage. Total movement in response to three light cycles (3 min of light to 3 min dark) was tracked in 96 wells during a 24-min assay. HD video was recorded at 15 frames/second.
