2.2.3. Histological Analysis

The osteogenesis effect of curcumin treatment in diabetic mice was evaluated by Masson's Trichrome staining (Beyotime). The sections were preserved in well-prepared Weigert's hematoxylin solution for 10 min. After rinsing with running tap water and acetified water, the slides were firstly stained by Masson Fuchsin Acid Complex for 5 min, then immersed in 2% glacial acetic acid solution, 1% phosphomolybdic acid, 2% aniline blue, and 0.2% acetic acid in sequence. Finally, sections were fixed into neutral gum for microscopy observation.
