**5. Conclusions**

Within the limitations of the current investigation, a topographic modification of the smooth surface of a metallic biomaterial showed reduction in bacterial adhesion. Three different topographies from natural leaves (*C. aurea*, *C. esculenta* and *S. molesta*) were used, but only surfaces based on *C. aurea* and *C. esculenta* models showed such effect, even though there was an increase in roughness after every transference. There were also a reduction in the hydrophobicity after the transference due to hierarchical features and protective coatings that soft lithography is incapable of transferring. The most hydrophobic surfaces showed higher bacterial adhesion, which is also related to the bacterial species used in the current investigation. The results in bacterial adhesion suggests a relation between roughness, hydrophobicity and topographic features that is relevant to reduce the adhesion of this bacterial species to the surface of SS. Such reduction is important in short term investigations because it opens new possibilities in the field of using materials and surfaces that nature has to offer to improve biomaterials for medical and dental applications. However, due to conflicting results obtained in the present work, careful attention must be given to the selection of the natural surface.

**Author Contributions:** Conceptualization, S.A.-S.; Data curation, J.S.-G. and J.F.; Formal analysis, J.S.-G. and J.F.; Investigation, S.A.-S. and L.S.; Methodology, S.A.-S. and L.S.; Project administration, S.A.-S.; Supervision, S.A.-S. and J.S.-G.; Writing—original draft, S.A.-S. and L.S.; Writing—review & editing, S.A.-S., J.S.-G. and J.F. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding author.

**Acknowledgments:** The authors would like to thank Claudia García from Universidad Nacional de Colombia for her assistance with the sol-gel method and Johanna Gutiérrez from Tecnoacademia for her assistance with the AFM.

**Conflicts of Interest:** The authors declare no conflict of interest.

#### **References**

