*4.3. Lentiviral Vector for Immortalizing Passaged Ovine Trophoblast Cells*

To immortalize the OTR cells, pLV-hTERT-IRES-hygro was used (Addgene, Watertown, MA, USA, Plasmid # 85140) [67]. The pLV-hTERT-IRES-hygro vector-based lentiviral particles expressed human telomerase reverse transcriptase (hTERT) in the infected cells.
