*4.6. Cell Growth Assay*

Cells were trypsinized and resuspended in PBS for cell number counting or they were centrifuged for further Western blot analysis after drug treatment. Centrifuged cells were further lysed with lysis buffer containing 0.5% NP-40, 300 mM NaCl, 1 mM EDTA, and the protease inhibitor cocktail (Roche, Mannheim, Germany) followed by centrifugation (15,000 rpm, 4 ◦C). The supernatant was collected and further analyzed by Western blot analysis.
