*4.4. Microtubule Regrowth Assay*

Cells were cultured in nocodazole (10 mg/mL) containing medium for 1 h to depolymerize the microtubules. Then, the nocodazole was removed by washing with PBS three times, and then cells were incubated in fresh medium at 37 ◦C for 10 min. After microtubule repolymerization, cells were fixed with cold methanol at −20 ◦C for 6 min, followed by immunofluorescence staining.
