*4.8. Statistics*

Statistical analyses were performed with Prism version 8.4.1 (GraphPad, La Jolla, CA, USA). We analyzed these data to identify outliers and removed all outliers prior to performing statistical comparisons. Normality was determined using the Shapiro–Wilk test. Significant differences between medians were determined by unpaired Mann–Whitney U test. For normally distributed data, significant differences between means were determined by unpaired *t* test and ANOVA. Binary data were compared using Fisher's exact test. Data are presented as median + IQR data or mean ± standard deviation (SD). Statistical significance was defined as *p* < 0.05.

**Supplementary Materials:** Supplementary Materials can be found at http://www.mdpi.com/1422-0067/21/18/ 6477/s1.

**Author Contributions:** Conception, C.J.S., J.K.K., N.C.D., N.M.M.; methodology, A.M.B., C.J.S., N.C.D., N.M.M., S.B.; validation, C.J.S., C.C.Y., N.C.D., N.M.M., T.W., V.O.; formal analysis, C.C.Y., N.C.D., N.M.M.; investigation, C.C.Y., N.C.D., N.M.M., N.V.-P., S.B., T.W., V.O.; resources, A.M.B., J.K.K., N.C.D.; writing—original draft preparation C.J.S., N.C.D., N.M.M., N.V.-P.; writing—review and editing C.J.S., J.K.K., N.C.D., N.M.M.; visualization, N.C.D., N.M.M.; supervision, A.M.B., C.J.S., J.K.K., N.C.D.; funding acquisition, N.C.D. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by National Institutes of Health, grant number: R01HL127013.

**Acknowledgments:** We thank Jason Butler and Ralf Adams for their donations of the experimental mice for generation of our colony. We thank Naiche Adler for her oversight on the development and layout of the project and figures. We thank Sara Morelli and Pranela Rameshwar who provided insightful comments and assistance with design of the project. All schematic representations were created with BioRender.com.

**Conflicts of Interest:** The authors declare no conflict of interest.
