*4.6. Tissue Collection*

For analysis of LIN28A or LIN28B knockdown and its effect on conceptus elongation, terminal surgeries were conducted on recipient ewes at 16 days gestational age (dGA), and tissues were collected. Conceptuses were flushed from the uterus using DMEM-F-12 (1:1) medium. After separating the embryo, trophectoderm length was measured, and both embryo and TE were snap frozen. TE samples were used to extract mRNA, miRNA, or proteins for further analysis.
