*4.12. Cloning*

Primers to amplify putative PLAGL1 enhancer regions were designed using the mm9 genome and cloned into the PGL4.23 vector using the ligation independent cloning method as previously described [107] with a modification. Target enhancer regions were amplified with NEB Q5 DNA polymerase (NEB M0491S) using primers listed in Supplemental Table S7. For each enhancer target, three colonies were selected to identify positive clones by colony PCR and sequenced with Applied Biosystems 3730xl DNA Analyzer (DNA Facility, Iowa State University).
