2.4.3. Microstructure

Commercial mayonnaises (MT1, MT2, MV) and freshly made emulsions (MRO, MSO, MLO, MCO) were observed under an Olympus FluoView 3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). All samples were prepared by adding 0.01% Nile red and analyzed using a diode 488 nm laser (excitation). The area of oil droplets (ODs) from each sample was quantified from 10 to 15 confocal images acquired by FluoView software (Olympus), corresponding to ∼1000 ODs by using ImageJ software (www.imagej.nih.gov) (accessed on 6 November 2020). Data point-box plots were generated with Origin Pro software (OriginLab Corporation, Northampton, MA, USA).
