*2.3. Extract Characterization*

### 2.3.1. Total Phenolic Content Determination

The total phenol content was determined according to the Folin–Ciocalteu method [43]. To 980 μL of H2O Milli-Q, 20 μL of appropriately diluted extract, 100 μL of Folin–Ciocalteu reagen<sup>t</sup> were added. After 3 min, 800 μL of 7.5% Na2CO3 were added and then the sample was stored in the dark for 60 min. The absorbance was read at 720 nm (Cary 60 UV-Vis, Agilent Technologies, Mulgrave, Australia). The results were expressed as mg of gallic acid equivalents (GAE) per g of dry weight sample (mg GAE g<sup>−</sup><sup>1</sup> DW). Each sample was analysed in duplicate.
