*2.3. Total Polyphenolic Content*

The total phenolic content (TPC) of the extracts was determined spectrophotometrically as described by Barbosa-Pereira et al. [13] using a BioTek Synergy HT spectrophotometric multi-detection 96-well microplate reader (BioTek Instruments, Winooski, VT, USA). The absorbance was measured in triplicate at 740 nm. A standard curve of gallic acid (100–600 μM; R<sup>2</sup> = 0.9994) was used to quantify the phenolic content, which was expressed as milligrams of gallic acid equivalents per milliliter of the fresh extract (mg GAE/mL).
