*2.6. Antiproliferative Activity Procedures*

The antiproliferative studies on HCT116 and MDA-MB-231 cell lines were conducted using the [3H]-thymidine incorporation assay method, as previously reported by [34]. Essentially, the method was conducted by seeding 3000 cells in each well of the 96-well plates with a solution of non-derivatized and derivatized extracts in dimethyl sulfoxide (DMSO) for 3 days. Then, [3H]-thymidine is added to the cells, and they are incubated for 6 h. The cells were then counted using a Trilux/Betaplate counter, which shows the percentage of the cells with [3H]-thymidine that are incorporated into the DNA helix. This

was conducted in duplicate. The antiproliferative activity was determined as cell growth percentages relative to the 100% growth in the control (non-treated).
