*2.8. Photochemiluminescence Assay*

An amount of 0.05 g of samples was mixed with 30 mL methanol for3h[32]. The extractions were performed in tightly closed plastic tubes, vortexed, and centrifuged at 20 ◦C. The extraction was made in triplicate. The scavenging activity of byproduct samples was evaluated by a photochemiluminescence (PCL-ACL) method in which superoxide radical anions (O2−) are generated from luminol. The extracts were dissolved in methanol. The reactions were carried out using kits for the determination of antioxidant capacity of lipid-soluble substances (Analytik Jena, Jena, Germany), mixing 2300 μL of methanol (reagent 1), 200 μL of buffer solution (reagent 2), 25 μL of luminol (reagent 3), and 10 μL of sample. Measurement was performed on a Photochem device with PCLsoft software (Analytik Jena). Trolox was used to prepare the calibration curve. The results are expressed as mg of Trolox equivalents per g of extract.
