**4. Conclusions**

Due to its higher content of nutrients compared to other species, ovine scotta is a precious substrate that can be valorized through a multidisciplinary biotechnological approach with the aim of producing ingredients with specific biological activities. The enzymatic hydrolyses performed both with bromelain and pancreatin on retentate of scotta allowed enhancement of its DPP-IV inhibitory and antioxidant activities, bromelain being more promising in such an aim. Likewise, the antibacterial activity of hydrolysates slightly increased with respect to control, even if an inhibitory effect against some Listeria monocytogenes strains of the non-hydrolysates scotta was also noticed. LC-MS/MS analysis allowed identification among the experimental groups of several differential peptides that contain sequences with known activities among those here studied. Further studies are needed to optimize reaction conditions, in order to maximize such biological activities in relation to the specific objective.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2304-815 8/10/12/3137/s1, Sheet S1: All identified peptides in BSPH, PSHP, and CTRL. Sheet S2: Differential proteins analysis of BSPH vs. CTRL. Sheet S3: Differential peptides analysis of BSPH vs. CTRL. Sheet S4: Differential proteins analysis of PSPH vs. CTRL. Sheet S5: Differential peptides analysis of PSPH vs. CTRL. Sheet S6: Differential proteins analysis of BSPH vs. PSPH. Sheet S7: Differential peptides analysis of BSPH vs. PSPH.

**Author Contributions:** Conceptualization, P.P.U.; methodology, P.P.U., R.C., F.F., G.L.P.; formal analysis, R.C., F.F., P.P.U., S.P.; investigation, R.C., F.F., R.A., S.P.; resources, P.P.U.; writing—original draft preparation, R.C., P.P.U., F.F., G.L.P.; writing—review and editing, P.P.U., R.C., G.L.P., F.F., D.P., S.P.; supervision, P.P.U. and A.P.; project administration, P.P.U.; funding acquisition, P.P.U. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by Regione Autonoma della Sardegna-Legge Regionale 7 agosto 2007, n.7, Bando Capitale Umano ad Alta Qualificazione, annualità 2015 and by Fondo dell'Ateneo di Sassari per la ricerca 2020.

**Data Availability Statement:** The peptides identified in this study were analyzed for potential activity with the tool available on BIOPEP-UWM database at the following link: https://biochemia. uwm.edu.pl/en/biopep-uwm-2/.

**Acknowledgments:** The authors would like to thank Nutraceutica SRL and Enzyme Development Corporation for the supply of enzymes, and Caseificio F.lli Pinna (Thiesi, SS) for the scotta samples used in this study.

**Conflicts of Interest:** The authors declare no conflict of interest.
