3.6.4. Anti-Inflammatory Assay

The zebrafish inflammation model was induced by CuSO4 to evaluate the effects of compounds on anti-inflammation [28]. In total, 72 hpf zebrafish larvae were distributed into 24-well plates (ten embryos per well) in a 2 mL final volume of culture water, and treated with different concentrations of each test compound (30, 70, 120 μg/mL) for 2 h as test group. Then CuSO4 was added and incubated for 1 h. The control group was fresh culture water, the model group was 20 μM CuSO4 and the positive drug group was 20 μM CuSO4 and 10 μM ibuprofen. After 4 h incubation in a light-operated incubator at 28.0 ◦C ± 0.5 ◦C, the number of macrophages were imaged by a fluorescent microscope (Olympus, SZX2-ILLTQ, Tokyo, Japan). All treatments were performed in triplicate.
