*Article* **New Prenylated Indole Homodimeric and Pteridine Alkaloids from the Marine-Derived Fungus** *Aspergillus austroafricanus* **Y32-2**

**Peihai Li 1,2,3,†, Mengqi Zhang 1,2,3,†, Haonan Li 1, Rongchun Wang 1,3, Hairong Hou 1,3, Xiaobin Li 1,3,\*, Kechun Liu 1,3,\* and Hao Chen 4,\***

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**Abstract:** Chemical investigation of secondary metabolites from the marine-derived fungus *Aspergillus austroafricanus* Y32-2 resulted in the isolation of two new prenylated indole alkaloid homodimers, di-6-hydroxydeoxybrevianamide E (**1**) and dinotoamide J (**2**), one new pteridine alkaloid asperpteridinate A (**3**), with eleven known compounds (**4**–**14**). Their structures were elucidated by various spectroscopic methods including HRESIMS and NMR, while their absolute configurations were determined by ECD calculations. Each compound was evaluated for pro-angiogenic, anti-inflammatory effects in zebrafish models and cytotoxicity for HepG2 human liver carcinoma cells. As a result, compounds **2**, **4**, **5**, **7**, **10** exhibited pro-angiogenic activity in a PTK787-induced vascular injury zebrafish model in a dose-dependent manner, compounds **7**, **8**, **10**, **11** displayed anti-inflammatory activity in a CuSO4-induced zebrafish inflammation model, and compound **6** showed significant cytotoxicity against HepG2 cells with an IC50 value of 30 μg/mL.

**Keywords:** marine-derived fungus; *Aspergillus austroafricanus*; novel bioactive metabolites; proangiogenesis; anti-inflammatory effects
