**4. Conclusions**

In summary, this work shows the analysis of avocado oil extracted from the Zutano variety by two (SE and UAE) techniques. In this context, petroleum ether volatile fractions were estimated over three ripening stages (breaking, ripe, and overripe) using HS-SPME–GC–MS. The Zutano variety, which is cultivated in the Crete region, gave a fragrant oil which has not been previously studied. This cultivar is characterized from seven main volatile compounds, including 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene (<sup>α</sup>pinene), 1-methyl-4-(prop-1-en-2-yl)cyclohex-1-ene (D-limonene), 4,10-dimethyl-7-propan-2-yltricyclo[4.4.0.01,5]dec-3-ene (α-cubebene), (1*S*,6*S*,7*S*,8*S*)-1,3-dimethyl-8-propan-2-yltricyclo[4.4.0.02,7]dec-3-ene (a-copaene), (1*R*,9*S*,*<sup>Z</sup>*)-4,11,11-trimethyl-8-methylenebicyclo[7.2.0]undec-4-ene (β-caryophyllene), 2,6-dimethyl-6-(4-methylpent-3-enyl)bicyclo[3.1.1]hept-2-ene (α-bergamotene), and (1*E*,4*E*,8*<sup>E</sup>*)-2,6,6,9-tetramethylcycloundeca-1,4,8-triene (humulene). The analyzed fractions consisted of a high content of terpenoids with an average relative abundance of over 61.7%. ANOVA revealed that the extraction methods did not have statistically significant differences between their qualification and semi-quantification results. In contrast, the application of MANOVA between the ripening stages and some volatiles indicated that the *p*-values were lower than 0.05. Even though ripening is one significant factor that affects volatiles, additional research is required to approve the above results. This study could form the foundation for additional research on the impact of ethylene and the metabolism of avocado oil volatiles.

**Author Contributions:** Conceptualization, M.X. and C.S.P.; methodology, M.X., L.V., E.H.K., P.-K.R. and C.S.P.; software, M.X., L.V., E.H.K. and P.-K.R.; validation, M.X. and E.G.; formal analysis, E.G.; investigation, M.X., E.G., L.V. and E.H.K.; resources, E.G.; data curation, M.X. and E.G.; writing—original draft preparation, M.X.; writing—review and editing, L.V., E.H.K., P.-K.R., C.S.P. and P.A.T.; visualization, C.S.P. and P.A.T.; supervision, C.S.P.; project administration, C.S.P. and

P.A.T.; funding acquisition, E.G. and P.-K.R. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** The authors would like to thank Ioannis Lathourakis and Antonios Galanis for the sponsorship of *Persea americana* Mill. "Zutano" variety avocados from the Greek island of Crete, Chania.

**Conflicts of Interest:** The authors declare no conflict of interest.
