*Open Field*

Besides its value as a test for locomotor activity and anxiety, the open field task provides information on habituation as a form of learning [37]. For this purpose, each mouse was placed into the center of a 28.5 × 29.8 cm box (in-house manufactured, Fraunhofer IME, Schmallenberg, Germany). Animals were allowed to explore the new environment for 30 min. Total distance moved and percentage of time spent in the inner zone of the box were automatically detected by camera tracking and corresponding EthoVision XT 13 software (Noldus, Wageningen, The Netherlands). Data were analyzed additionally for time blocks of 5 min.

#### *Elevated Zero Maze*

To investigate anxiety related behavior [78], we placed each animal into the open corridor of a 60 cm diametric elevated zero maze (Ugo Basile SRL, Gemonio, Italy) for a duration of 5 min. The maze consisted of two open and two closed 5 cm wide corridors. Besides the time spent in the open corridors, the total distance moved by the mice was automatically detected by camera tracking and corresponding EthoVision XT 13 software.
